ArticleTranslational lung cancer research2024
Correlations between 14-gene RNA-level assay and clinical and molecular features in resectable non-squamous non-small cell lung cancer: a cross-sectional study.
Article in Translational lung cancer research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Utilizing clinical features, genetic mutations, and a 14-gene molecular assay for optimizing the management of multiple primary lung cancer.Translational lung cancer research · 2026Article
- Distinct metabolic profiles in lung adenocarcinomas presenting as solid or ground-glass opacities.NPJ precision oncology · 2026Article
- Value of the 14-gene molecular assay in efficacy assessment of neoadjuvant chemoimmunotherapy for non-small cell lung cancer.Frontiers in molecular biosciences · 2025Article
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21 authors.
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Abstract
Background: Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide. Accurate risk stratification is essential for optimizing treatment strategies. A 14-gene RNA-level assay of lung cancer, which involves quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of formalin-fixed paraffin-embedded (FFPE) tissue samples, offers a promising approach. The aim of our study was to assess the relationships between risk stratification, as determined by a 14-gene RNA-level assay, and various clinical and molecular characteristics. Methods: We retrospectively collected the preoperative clinical information and molecular testing information from 102 resectable non-squamous NSCLC patients. The 14-gene RNA-level assay was performed by extracting RNA from FFPE samples, followed by reverse transcription and quantification via quantitative polymerase chain reaction (qPCR) to assess the expression levels of 11 cancer-associated genes and three housekeeping genes. These gene expression levels were used to calculate a risk score, enabling patient stratification into distinct risk groups. Based on the 14-gene risk stratification, we analyzed the correlations between the clinical and molecular characteristics across the high-, medium-, and low-risk groups. Results: A total of 102 patients were included in the study. The mean age was 55.19 years, 67 (65.7%) patients were female, and 18 (17.6%) had a smoking history. The 14-gene risk stratification classified patients into low-risk (n=63), intermediate-risk (n=25), and high-risk (n=14) groups. No significant differences were observed in baseline demographics between the three risk groups. High-risk patients had significantly higher mean computed tomography (CT) value (P=0.01) and enhanced CT value (P=0.02) compared to low-risk patients. Genomic profiling of 89 patients revealed specific mutations that were significantly associated with the higher-risk groups. Tumor mutational burden (TMB) was higher in higher-risk groups (P=0.007). In clinically low-risk patients (n=85) as recognized by the NCCN guidelines, the 14-gene risk stratification model reclassified 30 out from the 85 clinically low-risk patients, with 19 placed in the medium-risk group and 11 in the high-risk group, while the remaining samples were still classified as low-risk. Additionally, we found that three patients who were not recommended for adjuvant therapy by the Multiple-gene INdex to Evaluate the Relative benefit of Various Adjuvant therapies (MINERVA) model were classified as high risk and 13 as intermediate risk. Conclusions: Our results indicate that 14-gene RNA-level assay is correlated with specific genetic mutations, including
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