Evidence map›Paper›PMID 39674511›Full record

ArticleMolecular & cellular proteomics : MCP2025

Spatial Organization of the Sperm Cell Glycoproteome.

Rensong Ji, Riccardo Zenezini Chiozzi, Henk van den Toorn, Miguel Leung, Tzviya Zeev-Ben-Mordehai, Nathan D Burke, Elizabeth G Bromfield, Karli R Reiding, Albert J R Heck

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rensong JiBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands; Netherlands Proteomic Center, Utrecht, The Netherlands.
Riccardo Zenezini ChiozziBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands; Netherlands Proteomic Center, Utrecht, The Netherlands; Division of Biosciences, Institute of Structural and Molecular Biology, University College London, London, United Kingdom; Division of Biosciences, University College London Mass Spectrometry Science Technology Platform, University College London, London, United Kingdom.
Henk van den ToornBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands; Netherlands Proteomic Center, Utrecht, The Netherlands.
Miguel LeungStructural Biochemistry, Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.
Tzviya Zeev-Ben-MordehaiStructural Biochemistry, Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.
Nathan D BurkeSchool of BioSciences, Faculty of Science, Bio21 Institute, University of Melbourne, Parkville, VIC, Australia; Infertility and Reproduction Research Program, School of Environment and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Elizabeth G BromfieldSchool of BioSciences, Faculty of Science, Bio21 Institute, University of Melbourne, Parkville, VIC, Australia; Infertility and Reproduction Research Program, School of Environment and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia; Department of Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands.
Karli R ReidingBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands; Netherlands Proteomic Center, Utrecht, The Netherlands. Electronic address: k.r.reiding@uu.nl.
Albert J R HeckBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands; Netherlands Proteomic Center, Utrecht, The Netherlands. Electronic address: a.j.r.heck@uu.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sperm cells are terminally differentiated cells that are essential for reproduction in sexually reproducing species. Consistent with their highly specialized function, sperm cells harbor a unique proteome containing many proteins not expressed in somatic cells. In contrast, the post-translational landscape of the sperm proteome remains largely unexplored, limiting our understanding of how modifications such as glycosylation impact sperm function and sperm-egg interactions. Here, we used glycopeptide-centric glycoproteomics to comprehensively characterize protein N-glycosylation in sperm from three mammalian species, revealing clear conservation of glycosylation profiles. We find that glycosylation patterns in sperm proteins are distinct from those in plasma, with as clear distinctive features less sialyation and more paucimannosylation in sperm. Moreover, based on their subcellular location, sperm protein glycosylation varies, with paucimannose species enriched in the acrosomal vesicle, oligomannose species in the sperm head membrane, and complex glycan species in the acrosomal membrane.

Indexed as

GlycoproteinsProteomeSpermatozoaAcrosomeAnimalsGlycopeptidesGlycosylationMaleMiceProtein Processing, Post-TranslationalProteomicsGlycopeptidesGlycoproteinsProteome

Identifiers

PMID39674511
PMCPMC11774830

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.