Evidence map›Paper›PMID 39678532›Full record

ArticleFrontiers in neuroscience2024

Extrinsic electric field modulates neuronal development and increases photoreceptor population in retinal organoids.

Deepthi S Rajendran Nair, Anika Gupta, Ege Iseri, Tianyuan Wei, Le Tam Phuong Quach, Magdalene J Seiler, Gianluca Lazzi, Biju B Thomas

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in neuroscience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Deepthi S Rajendran NairDepartment of Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Anika GuptaDepartment of Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Ege IseriDepartment of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, United States.
Tianyuan WeiDepartment of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, United States.
Le Tam Phuong QuachDepartment of Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Magdalene J SeilerDepartments of Physical Medicine and Rehabilitation; Ophthalmology; Anatomy and Neurobiology, University of California, Irvine, Irvine, CA, United States.
Gianluca LazziDepartment of Electrical and Computer Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, United States.
Biju B ThomasDepartment of Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.

Funding

Chinese American Eye StudyU10EY017337 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI VARMA, ROHIT · 2009 to 2013
$10.2M
Ophthalmic Therapeutics Engineering CoreP30EY029220 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Mahnaz Shahidi · 2018 to 2026
$6.5M
Integration and functionality of retinal organoid transplantsR01EY031834 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI SEILER, MAGDALENE J · 2021 to 2025
$3.0M
VRC: A Stem Cell-Based Treatment Strategy for Laser-Induced Permanent Retinal DamagesR01EY031144 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI THOMAS, BIJU BLAVADY · 2019 to 2021
$751k
NEI NIH HHS P30 EY029220NEI NIH HHS R01 EY031144NEI NIH HHS R01 EY031834NEI NIH HHS U10 EY017337
6 · The paper itself

Abstract

Introduction: Considering the significant role played by both intrinsic and extrinsic electric fields in the growth and maturation of the central nervous system, the impact of short exposure to external electric fields on the development and differentiation of retinal organoids was investigated. Methods: Retinal organoids derived from human embryonic stem cells were used at day 80, a key stage in their differentiation. A single 60-minute exposure to a biphasic electrical field was administered to assess its influence on retinal cell populations and maturation markers. Immunohistochemistry, qPCR, and RNA sequencing were employed to evaluate cell type development and gene expression changes. Results: Electrical stimulation significantly enhanced neuronal development and increased the population of photoreceptors within the organoids. RNA sequencing data showed upregulated expression of genes related to rod photoreceptors, Müller cells, horizontal cells, and amacrine cells, while genes associated with retinal pigment epithelium and retinal ganglion cells were downregulated. Variations in development and maturation were observed depending on the specific parameters of the applied electric field. Discussion: These findings highlight the significant impact of extrinsic electrical fields on early retinal development and suggest that optimizing electrical field parameters could effectively address certain limitations in retinal organoid technology, potentially reducing the reliance on chemicals and small molecules.

Indexed as

electrical fieldelectrical stimulationphotoreceptorsretinal degenerationretinal organoidsstem cell differentiation

Identifiers

PMID39678532
PMCPMC11639233

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.