Evidence map›Paper›PMID 39682739›Full record

ArticleCells2024

Transcriptomic Analysis of Air-Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation.

Jieun Kim, Eun-Young Eo, Bokyong Kim, Heetak Lee, Jihoon Kim, Bon-Kyoung Koo, Hyung-Jun Kim, Sukki Cho, Jinho Kim, Young-Jae Cho

Erratum issuedAbstract read
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Airway epithelial heterogeneity and mucus plugging in asthmatic bronchioles.American journal of respiratory and critical care medicine · 2026
    Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Scaling Large Language Models for Next-Generation Single-Cell Analysis.bioRxiv : the preprint server for biology · 2026
    Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. Review
  13. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Jieun KimDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.ORCID 0009-0001-8992-4060
Eun-Young EoDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
Bokyong KimDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.ORCID 0000-0002-1326-5206
Heetak LeeCenter for Genome Engineering, Institute for Basic Science, Daejeon 34126, Republic of Korea.
Jihoon KimCenter for Genome Engineering, Institute for Basic Science, Daejeon 34126, Republic of Korea.ORCID 0000-0002-3258-6953
Bon-Kyoung KooCenter for Genome Engineering, Institute for Basic Science, Daejeon 34126, Republic of Korea.
Hyung-Jun KimDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.ORCID 0000-0003-1984-8864
Sukki ChoDepartment of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
Jinho KimDepartment of Genomic Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
Young-Jae ChoDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.ORCID 0000-0001-6943-4462

Funding

National Research Foundation of Korea 2021K1A4A7A02097757Seoul National University Bundang Hospital research fund No. 02-2023-0049
6 · The paper itself

Abstract

To develop in vitro respiratory models, it is crucial to identify the factors involved in epithelial cell differentiation. In this study, we comprehensively analyzed the effects of air-liquid interface (ALI) culture on epithelial cell differentiation using single-cell RNA sequencing (scRNA-seq). ALI culture induced a pronounced shift in cell composition, marked by a fivefold increase in ciliated cells and a reduction of more than half in basal cells. Transcriptional signatures associated with epithelial cell differentiation, analyzed using iPathwayGuide software, revealed the downregulation of

Indexed as

Cell DifferentiationEpithelial CellsGene Expression ProfilingLungOrganoidsCell Culture TechniquesHumansTranscriptomeVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Aair–liquid interfaceairway epitheliumciliated cellsdifferentiationsingle-cell RNA-sequencing

Identifiers

PMID39682739
PMCPMC11639892

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.