Evidence mapPaperPMID 39687022Full record

ArticleiScience2024

CFTR represses a PDX1 axis to govern pancreatic ductal cell fate.

Pavana G Rotti, Yaling Yi, Grace Gasser, Feng Yuan, Xingshen Sun, Idil Apak-Evans, Peipei Wu, Guangming Liu, Soon Choi, Rosie Reeves and 10 more

Abstract read
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Pavana G RottiWhitehead Institute, MIT, Cambridge, MA, USA.
Yaling YiDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Grace GasserDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Feng YuanDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Xingshen SunDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Idil Apak-EvansDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Peipei WuDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Guangming LiuDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Soon ChoiDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Rosie ReevesDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Attilina E ScioneauxDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Yulong ZhangDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Michael WinterDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Bo LiangDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Nathan CunicelliDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Aliye UcStead Family Department of Pediatrics, Carver College of Medicine, Iowa City, IA, USA.
Andrew W NorrisCenter for Gene Therapy, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Lori SusselBarbara Davis Center for Childhood Diabetes, University of Colorado Anschutz, Medical Campus, Aurora, CO, USA.
Kristen L WellsBarbara Davis Center for Childhood Diabetes, University of Colorado Anschutz, Medical Campus, Aurora, CO, USA.
John F EngelhardtDepartment of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.

Funding

Pulmonary Toxicology FacilityP30ES005605 · UNIVERSITY OF IOWA · 1990 to 2025
$8.8M
TARGETED MODULATION OF VASOPRESSIN IN BRAIN MAGNOCELLULAR NEURONS BY GENE THERAPYP30DK054759 · UNIVERSITY OF IOWA · 1998 to 2025
$7.4M
NIDDK NIH HHS P30 DK054759NIEHS NIH HHS P30 ES005605
6 · The paper itself

Abstract

Inflammation, acinar atrophy, and ductal hyperplasia drive pancreatic remodeling in newborn cystic fibrosis (CF) ferrets lacking a functional cystic fibrosis conductance regulator (CFTR) channel. These changes are associated with a transient phase of glucose intolerance that involves islet destruction and subsequent regeneration near hyperplastic ducts. The phenotypic changes in CF ductal epithelium and their impact on islet function are unknown. Using bulk RNA sequencing (RNA-seq), single-cell RNA sequencing (scRNA-seq), and assay for transposase-accessible chromatin using sequencing (ATAC-seq) on CF ferret models, we demonstrate that ductal CFTR protein constrains PDX1 expression by maintaining PTEN and GSK3β activation. In the absence of CFTR protein, centroacinar cells adopted a bipotent progenitor-like state associated with enhanced WNT/β-Catenin, transforming growth factor β (TGF-β), and AKT signaling. We show that the level of CFTR protein, not its channel function, regulates PDX1 expression. Thus, this study has discovered a cell-autonomous CFTR-dependent mechanism by which

Indexed as

Cell biologyMolecular biologyPhysiologyTranscriptomics

Identifiers

PMID39687022
PMCPMC11647141

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.