Evidence map›Paper›PMID 39688410›Full record

ArticlemBio2025

Split-Cre-mediated GFP expression as a permanent marker for flagellar fusion of

Ruth Etzensperger, Mattias Benninger, Berta Pozzi, Ruth Rehmann, Arunasalam Naguleswaran, Gabriela Schumann, Jan Van Den Abbeele, Isabel Roditi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ruth EtzenspergerInstitute of Cell Biology, University of Bern, Bern, Switzerland.ORCID 0009-0004-9455-4465
Mattias BenningerInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Berta PozziInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Ruth RehmannInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Arunasalam NaguleswaranInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Gabriela SchumannInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Jan Van Den AbbeeleDepartment of Biomedical Sciences, Trypanosoma Unit, Institute of Tropical Medicine, Antwerp, Belgium.
Isabel RoditiInstitute of Cell Biology, University of Bern, Bern, Switzerland.ORCID 0000-0003-2812-6513

Funding

Howard Hughes Medical Institute (HHMI) 55007650Kanton Bern (Berne)
6 · The paper itself

Abstract

Trypanosomes have different ways of communicating with each other. While communication via quorum sensing, or by the release and uptake of extracellular vesicles, is widespread in nature, the phenomenon of flagellar fusion has only been observed in IMPORTANCE: We have established a procedure to permanently label pairs of trypanosomes that transiently fuse their flagella and exchange proteins. When this occurs, a reporter gene is permanently flipped from the "off" to the "on" position, resulting in the production of green fluorescent protein. Crucially, green trypanosomes can be detected in tsetse flies co-infected with the two cell lines, proving that flagellar fusion occurs in the host. To our knowledge, we are the first to describe a split-Cre-Lox system for lineage tracing and selection in trypanosomes. In addition to its use in trypanosomes, this system could be adapted for other parasites and in other contexts. For example, it could be used to determine whether flagellar fusion occurs in related parasites such as Leishmania and

Indexed as

FlagellaGreen Fluorescent ProteinsIntegrasesTrypanosoma brucei bruceiTsetse FliesAnimalsCre recombinaseGreen Fluorescent ProteinsIntegrasesflagellar fusionlineage tracingSplit-Cretrypanosomatsetse

Identifiers

PMID39688410
PMCPMC11796343

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.