Evidence mapPaperPMID 39695839Full record

ArticleEuropean journal of medical research2024

Comprehensive analysis of lysine lactylation and its potential biological significance in clear cell renal cell carcinoma.

Bangbei Wan, Yuan Huang, Binghao Gong, Yaohui Zeng, Cai Lv

Abstract read
In one paragraph

Article in European journal of medical research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Frontiers in cellular and infection microbiology · 2026
    Article
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bangbei Wan *Department of Urology, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China. 939313612@qq.com.
Yuan Huang *Department of Neurology, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China.
Binghao GongDepartment of Urology, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China.
Yaohui ZengDepartment of Urology, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China.
Cai LvDepartment of Urology, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China. 198312170@csu.edu.cn.

Funding

Hainan Province Clinical Medical Center QWYH202175Key R&D Projects of Hainan Province ZDYF2022SHFZ074National Natural Science Foundation of China 82160544Natural Science Foundation of Hainan Province 820RC771Research and Cultivation Fund of Hainan Medical University HYPY2020015Specific Research Fund of The Innovation Platform for Academicians of Hainan Province YSPTZX202311
6 · The paper itself

Abstract

backgroundClear cell renal cell carcinoma (ccRCC) is a common histological subtype of malignant renal neoplasm. Protein lysine lactylation (Kla) plays a crucial role in tumor metabolic reprogramming. However, little is known regarding the distribution and potential biological functions of Kla in ccRCC. This study aimed to systematically investigate the role of Kla in ccRCC.

methodsA total of 12 ccRCC samples were collected from 6 patients. Western blotting was performed to determine the trend of Kla-modified proteins in ccRCC. Liquid chromatography-tandem mass spectrometry was used to quantitatively analyze Kla in ccRCC. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) network analyses were conducted to clarify the biological functions and interactional relationships of differentially lactylated proteins (DLPs).

resultsIn total, 239 DLPs, including 441 lactylated sites, were identified by comparing ccRCC tissues with adjacent normal tissues. Kla-related enzymes have a higher affinity for alanine than for other amino acid residues in ccRCC. Subcellular localization analysis revealed that most DLPs were localized in the cytoplasm and mitochondria. GO enrichment analysis showed that most of the DLPs were enriched in metabolism-associated biological processes, including the purine ribonucleotide, monocarboxylic acid, ribonucleoside triphosphate, purine nucleoside triphosphate, and ATP metabolic processes. KEGG analysis indicated that most DLPs were also enriched in metabolism-related pathways, including glycolysis, amino acid (valine, leucine, and isoleucine) degradation, pyruvate metabolism, fatty acid degradation, and the citrate cycle. The top 20 hub proteins were screened from the PPI network based on their degree ranks.

conclusionsThis study revealed the role of Kla in ccRCC, which will extend our understanding of the potential molecular mechanisms underlying ccRCC formation and progression. These key Kla-modified proteins may be promising therapeutic targets for the treatment of ccRCC. However, further molecular experiments are required to validate these findings.

Indexed as

Carcinoma, Renal CellKidney NeoplasmsLysineFemaleHumansLipoylationMaleMiddle AgedProtein Interaction MapsLysineBioinformaticsClear cell renal cell carcinomaGene ontologyKyoto encyclopedia of genes and genomesLysine lactylation

Identifiers

PMID39695839
PMCPMC11657551

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.