Evidence map›Paper›PMID 39701053›Full record

ArticleJournal of vascular research2025

A Novel in vivo Rat Mesentery Model for Studying Tumor Spheroid-Induced Microvascular Remodeling.

Arinola O Lampejo, Luciana Fonseca Perez, Miriam M Girgis, Blanka Sharma, Dietmar W Siemann, Walter L Murfee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of vascular research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Arinola O LampejoJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, University of Florida Health Cancer Center, Gainesville, Florida, USA.
Luciana Fonseca PerezJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, University of Florida Health Cancer Center, Gainesville, Florida, USA.
Miriam M GirgisJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, University of Florida Health Cancer Center, Gainesville, Florida, USA.
Blanka SharmaJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, University of Florida Health Cancer Center, Gainesville, Florida, USA.
Dietmar W SiemannDepartment of Radiation Oncology, University of Florida, University of Florida Health Cancer Center, Gainesville, Florida, USA.
Walter L MurfeeJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, University of Florida Health Cancer Center, Gainesville, Florida, USA.

Funding

Team-based Interdisciplinary Cancer Research Training ProgramT32CA257923 · NCI · UNIVERSITY OF FLORIDA · PI SIEMANN, DIETMAR W · 2021 to 2025
$1.2M
Bioengineering Approach for Advancing Reparative Medicine Stem Cell TechnologiesR21HL159501 · NHLBI · UNIVERSITY OF FLORIDA · PI MURFEE, WALTER L · 2022 to 2023
$415k
NCI NIH HHS T32 CA257923NHLBI NIH HHS R21 HL159501
6 · The paper itself

Abstract

introductionThe tumor microenvironment is comprised of neoplastic cells and a variety of host cell types. Investigation of cell dynamics within this environment has motivated in vitro and ex vivo biomimetic model development. Our laboratory recently introduced the tumor spheroid-rat mesentery culture model to investigate cancer-induced lymphatic/blood vessel remodeling. To validate the physiological relevance of this model, the objective of this study was to determine the effect of tumor spheroids on microvascular remodeling after transplantation onto rat mesenteric tissues in vivo.

methodsSpheroids derived from H1299 lung cancer cells were seeded onto rat mesenteric tissues during a survival surgical procedure. Tissues were harvested 3-5 days post-seeding and stained with PECAM and LYVE-1 to identify blood and lymphatic vessels, respectively.

resultsAt all timepoints, cancer cells remained adhered to the tissue. Tissues seeded with tumor spheroids were shown to have increased vascular density, capillary sprouting, and tortuosity compared to sham tissues exposed to sterile saline only. Tumor spheroids also induced the formation of lymphatic/blood vessel connections and LYVE-1-negative protrusions emerging from lymphatic vessels.

conclusionOverall, this study underscores the use of in vivo modeling to aid in the discovery of novel vascular growth dynamics and offers new methodologies for studying tumor-induced remodeling.

introductionThe tumor microenvironment is comprised of neoplastic cells and a variety of host cell types. Investigation of cell dynamics within this environment has motivated in vitro and ex vivo biomimetic model development. Our laboratory recently introduced the tumor spheroid-rat mesentery culture model to investigate cancer-induced lymphatic/blood vessel remodeling. To validate the physiological relevance of this model, the objective of this study was to determine the effect of tumor spheroids on microvascular remodeling after transplantation onto rat mesenteric tissues in vivo.

methodsSpheroids derived from H1299 lung cancer cells were seeded onto rat mesenteric tissues during a survival surgical procedure. Tissues were harvested 3-5 days post-seeding and stained with PECAM and LYVE-1 to identify blood and lymphatic vessels, respectively.

resultsAt all timepoints, cancer cells remained adhered to the tissue. Tissues seeded with tumor spheroids were shown to have increased vascular density, capillary sprouting, and tortuosity compared to sham tissues exposed to sterile saline only. Tumor spheroids also induced the formation of lymphatic/blood vessel connections and LYVE-1-negative protrusions emerging from lymphatic vessels.

conclusionOverall, this study underscores the use of in vivo modeling to aid in the discovery of novel vascular growth dynamics and offers new methodologies for studying tumor-induced remodeling.

Indexed as

Lung NeoplasmsLymphangiogenesisLymphatic VesselsMesenteryMicrovesselsNeovascularization, PathologicSpheroids, CellularTumor MicroenvironmentVascular RemodelingAnimalsCell Line, TumorDisease Models, AnimalHumansMalePlatelet Endothelial Cell Adhesion Molecule-1RatsPlatelet Endothelial Cell Adhesion Molecule-1AngiogenesisLymphatic/blood vessel plasticityLymphaticsMicrovascular remodelingTumor

Identifiers

PMID39701053
PMCPMC11965820

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.