Evidence map›Paper›PMID 39704944›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Expression, Purification, and In Vitro Analysis of Myosin.

Glenn Carrington, Yasuharu Takagi, Oliver Umney, Alistair Curd, Jonathan E Bird, Michelle Peckham

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Glenn CarringtonAstbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.
Yasuharu TakagiLaboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Oliver UmneyAstbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.
Alistair CurdAstbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.
Jonathan E BirdDepartment of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.
Michelle PeckhamAstbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK. m.peckham@leeds.ac.uk.

Funding

Molecular Mechanisms of Hair Bundle Development and MaintenanceR01DC018827 · NIDCD · UNIVERSITY OF FLORIDA · PI Jonathan Edward Bird · 2020 to 2026
$3.0M
Studies Of Myosin VZIAHL004229 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI SELLERS, JAMES · 2009 to 2025
$2.3M
BBSRC BB/X017796/1Intramural NIH HHS ZIA HL004229NIDCD NIH HHS R01 DC018827
6 · The paper itself

Abstract

To understand the mechanics and kinetic properties of cytoskeletal molecular motors such as myosin, typically the motor of interest needs to be expressed and purified and then analyzed using a range of in vitro-based assays. In this chapter, we describe how to express and purify myosin using the insect cell system, how to characterize the purified protein by mass photometry and negative-stain EM to assess its quality, and how to perform in vitro assays in which fluorescently labeled myosin walks along actin tracks, including a brief description of adapting these assays for MINFLUX imaging.

Indexed as

MyosinsActinsAnimalsGene ExpressionSf9 CellsActinsMyosinsActinIn vitro motilityMass photometryMyosinNegative-stain electron microscopy

Identifiers

PMID39704944
PMCPMC11964125

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.