Evidence map›Paper›PMID 39707174›Full record

ArticleBMC plant biology2024

Transcriptome profiling of foxtail millet (Setaria italica) pollen and anther.

Dan-Ying Chen, Min Su, Huashuang Wu, Rui Zhao, Dan Wang, Shuqi Dong, Xiangyang Yuan, Xiaorui Li, Lulu Gao, Guanghui Yang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Dan-Ying Chen *College of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Min Su *College of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Huashuang WuCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Rui ZhaoCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Dan WangCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Shuqi DongCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Xiangyang YuanCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Xiaorui LiCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Lulu GaoCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Guanghui YangCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Xiaoqian ChuCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China. chuxiaoqian@sxau.edu.cn.
Jia-Gang WangCollege of Agriculture, Shanxi Agricultural University, Taigu, 030801, China. wjg@sxau.edu.cn.

Funding

National Key Research and Development Program of China 2023YFD1202702National Natural Science Foundation 32200222Shanxi Province college students innovation and Entrepreneurship training program 20220155Start-up Fund of Shanxi Agricultural University 2021BQ84
6 · The paper itself

Abstract

Pollen development and germination play a crucial role in the sexual reproduction of plants. This study analysis of transcriptional dynamics of foxtail millet pollen with other tissues and organs (ovule, glume, seedling and root) through RNA-sequencing revealed that a total of 940 genes were up-regulated in foxtail millet pollen. Based on this, we analyzed the genes involved in pollen tube growth of receptor kinases and small peptides, calcium signaling, small G proteins, vesicle transport, cytoskeleton, cell wall correlation, and transcription factors that are up-regulated in pollen. At the same time, we compared the gene expression of foxtail millet pollen and mature anthers, and found that a large number of transcription factors were specific expressed in mature anthers. In addition, we verified the accuracy of the transcriptome data using RT-qPCR. Finally, employed the antisense Oligonucleotide (as-ODN) system found that inhibiting SiPME67 expression would cause abnormal growth of pollen tube subapical. In summary, we preliminarily analyzed the genes that were up-regulated in foxtail millet pollen, which provided a reference for understanding the male sterility mechanism of foxtail millet in the future and theoretical basis for creating new male sterility lines.

Indexed as

Gene Expression ProfilingPollenSetaria PlantFlowersGene Expression Regulation, PlantGenes, PlantPlant ProteinsTranscriptomePlant Proteinsas-ODNFoxtail milletPollenPollen tubeTranscriptome

Identifiers

PMID39707174
PMCPMC11660619

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.