Evidence map›Paper›PMID 39708282›Full record

ArticleForensic toxicology2025

Benzoylmesaconine alters the native structure and activity of hen egg white lysozyme: revealing possible mechanism of aconitum-induced toxicity.

Manka Marycleopha, Jennifer Johnson, Abhishek Singh, Satish Kumar

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Article in Forensic toxicology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Manka MarycleophaLaboratory of Forensic Biology and Biotechnology, School of Forensic Science, National Forensic Science University, Gandhinagar, Gujarat, 382007, India.
Jennifer JohnsonLaboratory of Forensic Biology and Biotechnology, School of Forensic Science, National Forensic Science University, Gandhinagar, Gujarat, 382007, India.
Abhishek SinghNational Forensic Sciences University, Goa Campus, Ponda, Goa, 403401, India.
Satish KumarLaboratory of Forensic Biology and Biotechnology, School of Forensic Science, National Forensic Science University, Gandhinagar, Gujarat, 382007, India. satish.kumar@nfsu.ac.in.ORCID 0000-0002-2993-3694

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study examines the interaction between benzoylmesaconine (BMA) and hen egg white lysozyme (HEWL) under various physiological conditions, aiming to determine how BMA affects the HEWL's structure and function.

methodsSeveral analytical techniques were used, including tryptophan assay, light scattering, thioflavin T (ThT)-binding assay, dynamic light scattering, 8-anilino-1-naphthalenesulfonic acid (ANS)-binding assay, circular dichroism (CD) spectroscopy, enzyme activity assay, and molecular docking.

resultsThe tryptophan assay displayed a concentration-dependent decrease in tryptophan fluorescence, showing an interaction between BMA and HEWL. Light scattering and ThT-binding assays confirmed increased protein aggregation and amyloid fibril formation, while the ANS-binding assay demonstrated altered exposed hydrophobic regions, implying structural changes. CD spectroscopy showed a reduction in α-helix content, indicating conformational alterations, and enzyme activity assays showed a loss of lytic function due to structural distortion. Finally, molecular docking identified significant bonds and hydrophobic interactions between BMA and HEWL residues.

conclusionsBMA binding induces structural changes in proteins, forming small oligomers and amyloid fibrils that decrease HEWL enzymatic activity and disrupt functional integrity.

Indexed as

MuramidaseAnilino NaphthalenesulfonatesAnimalsBenzothiazolesChickensCircular DichroismMolecular Docking SimulationAnilino NaphthalenesulfonatesBenzothiazoleshen egg lysozymeMuramidaseAconitumAlkaloidsChronic toxicityHen egg white lysozymeNeurotoxicity

Identifiers

PMID39708282

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.