Evidence mapPaperPMID 39714986Full record

ArticleAmerican journal of physiology. Endocrinology and metabolism2025

The mitochondrial lactate oxidation complex: endpoint for carbohydrate carbon disposal.

Robert G Leija, Jose A Arevalo, Dianna Xing, José Pablo Vázquez-Medina, George A Brooks

Abstract read
In one paragraph

Article in American journal of physiology. Endocrinology and metabolism, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Resilience of the mitochondrial reticulum in aging.American journal of physiology. Endocrinology and metabolism · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Robert G LeijaExercise Physiology Laboratory, Department of Integrative Biology, University of California, Berkeley, California, United States.ORCID 0000-0002-7862-293X
Jose A ArevaloExercise Physiology Laboratory, Department of Integrative Biology, University of California, Berkeley, California, United States.ORCID 0000-0002-1120-4960
Dianna XingVazquez-Medina Lab, Department of Integrative Biology, University of California, Berkeley, California, United States.
José Pablo Vázquez-MedinaVazquez-Medina Lab, Department of Integrative Biology, University of California, Berkeley, California, United States.ORCID 0000-0003-1014-5214
George A BrooksExercise Physiology Laboratory, Department of Integrative Biology, University of California, Berkeley, California, United States.ORCID 0000-0002-1389-1629

Funding

The Biology of Peroxiredoxin 6R35GM146951 · UNIVERSITY OF CALIFORNIA BERKELEY · 2025 to 2025
$479k
HHS | NIH | National Institute on Aging (NIA) AG059715-01NIA NIH HHS R01 AG059715NIGMS NIH HHS R35 GM146951University of California Berkeley (UCB) 001
6 · The paper itself

Abstract

The lactate shuttle concept has revolutionized our understanding and study of metabolism in physiology, biochemistry, intermediary metabolism, nutrition, and medicine. Seminal findings of the mitochondrial lactate oxidation complex (mLOC) elucidated the architectural structure of its components. Here, we report that the mitochondrial pyruvate carrier (mPC) is an additional member of the mLOC in mouse muscle and C2C12 myoblasts and myotubes. Immunoblots, mass spectrometry, and co-immunoprecipitation experiments of mitochondrial preparations revealed abundant amounts of mitochondrial lactate dehydrogenase (mLDH), monocarboxylate transporter (mMCT), basigin (CD147), cytochrome oxidase (COx), and pyruvate carriers 1 and 2 (mPC1 and 2). In addition, using confocal laser scanning microscopy (CLSM) and in situ proximity ligation, we also demonstrated planar and three-dimensional (3-D) colocalization of pyruvate and lactate transporters with COx in fixed mouse skeletal muscle sections and C2C12 myoblasts and myotubes skeletal muscle sections, mouse muscle and C2C12 myoblasts and myotubes myotubes, and C2C12 myoblasts. This work serves as a landmark for configuring the final pathway of carbohydrate oxidation.

Indexed as

Electron Transport Complex IVLactic AcidMonocarboxylic Acid TransportersMuscle, SkeletalOxidation-ReductionAnimalsBasiginCarbohydrate MetabolismCarbonCell LineL-Lactate DehydrogenaseMaleMiceMice, Inbred C57BLMitochondriaMitochondrial Membrane Transport ProteinsBasiginBsg protein, mouseCarbonElectron Transport Complex IVLactic AcidL-Lactate DehydrogenaseMitochondrial Membrane Transport ProteinsMonocarboxylic Acid Transporterslactatelactate shuttlemitochondrial reticulumpyruvateskeletal muscle

Identifiers

PMID39714986
PMCPMC12145959

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.