Evidence mapPaperPMID 39716018Full record

ArticleThe journal of obstetrics and gynaecology research2025

Relationship between relaxin-2 levels in serum and mode of conception in twin gestations: A prospective cohort study.

Angela Silvano, Oumaima Ammar, Astrid Parenti, Noemi Strambi, Viola Seravalli, Daniele Bani, Mariarosaria Di Tommaso

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Article in The journal of obstetrics and gynaecology research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Angela SilvanoDepartment of Health Sciences, Division of Obstetrics and Gynecology, Careggi Hospital, University of Florence, Florence, Italy.
Oumaima AmmarDepartment of Health Sciences, Division of Obstetrics and Gynecology, Careggi Hospital, University of Florence, Florence, Italy.
Astrid ParentiDepartment of Health Sciences, Clinical Pharmacology and Oncology Section, University of Florence, Florence, Italy.
Noemi StrambiDepartment of Health Sciences, Division of Obstetrics and Gynecology, Careggi Hospital, University of Florence, Florence, Italy.
Viola SeravalliDepartment of Health Sciences, Division of Obstetrics and Gynecology, Careggi Hospital, University of Florence, Florence, Italy.
Daniele BaniDepartment of Clinical and Experimental Medicine, Research Unit of Human Histology & Embryology, University of Florence, Florence, Italy.
Mariarosaria Di TommasoDepartment of Health Sciences, Division of Obstetrics and Gynecology, Careggi Hospital, University of Florence, Florence, Italy.ORCID https://orcid.org/0000-0001-8248-929X

Funding

Fondazione CRF Firenze B15F21003630007
6 · The paper itself

Abstract

aimRelaxin is a peptide hormone commonly associated with pregnancy when it is thought to play a role in modulating various physiological processes to optimize maternal-fetal adaptation. In twin pregnancies these adaptive requirements are higher than in singleton pregnancies, therefore it is important to understand how circulating relaxin behaves in such conditions. This prospective cohort study aims to determine the serum relaxin-2 levels throughout gestation in twin pregnancies and to investigate its association with the mode of conception.

methodsBlood samples were collected during each trimester of gestation from 26 women with twin pregnancies obtained through spontaneous conception (SC, n = 18) or assisted reproductive technologies, specifically through intracytoplasmic sperm injection (ART, n = 8). Serum relaxin-2 levels were measured by a highly sensitive ELISA method.

resultsThe results indicated that serum relaxin-2 level peaks in the first trimester (491.05 ± 207.41 pg/mL), then decreases in the second trimester (446.27 ± 180.4 pg/mL; p < 0.057) and in the third trimester (422.19 ± 194.30 pg/mL; p < 0.025). Interestingly, the serum relaxin-2 level was higher in the spontaneous conception group with respect to the assisted reproductive technologies group (p < 0.001), when measured at each trimester of gestation. In addition, the multivariate regression analysis showed that only the assisted reproductive technologies had a significant impact on serum levels of relaxin-2 (p < 0.001), and no significant association was found with other women's clinical and demographic characteristics.

conclusionsThese findings extend the current knowledge about the pattern of circulating relaxin-2 throughout gestation in twin pregnancies, providing a sensitive measurement of serum relaxin-2 levels and a description of its putative physiological roles in humans.

Indexed as

Pregnancy, TwinRelaxinAdultFemaleFertilizationHumansPregnancyProspective StudiesRelaxinRLN2 protein, humanassisted reproductive technologiesmaternal and neonatal outcomesrelaxinspontaneous conceptiontwin pregnancies

Identifiers

PMID39716018
PMCPMC11666884

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