Evidence map›Paper›PMID 39721032›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

SPP1-ITGα5/β1 Accelerates Calcification of Nucleus Pulposus Cells by Inhibiting Mitophagy via Ubiquitin-Dependent PINK1/PARKIN Pathway Blockade.

Hanwen Gu, Qi Li, Zhenchuan Liu, Yanlin Li, Kaiwen Liu, Xiangzhen Kong, Yuanqiang Zhang, Qunbo Meng, Kangle Song, Qing Xie and 2 more

Abstract read
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
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  10. Single-cell Profiling Reveals Cooperative Participation ofInternational journal of medical sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hanwen GuDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Qi LiDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Zhenchuan LiuDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Yanlin LiDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Kaiwen LiuDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Xiangzhen KongDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Yuanqiang ZhangDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Qunbo MengDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Kangle SongDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Qing XieDepartment of Pharmacy, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
Yuan GaoDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Lei ChengDepartment of Orthopedic, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.ORCID https://orcid.org/0000-0003-0856-5359

Funding

National Natural Science Foundation of China 82202752National Natural Science Foundation of China 82272548Natural Science Foundation of Shandong Province ZR202110310011Natural Science Foundation of Shandong Province ZR2023MH194
6 · The paper itself

Abstract

Low back pain (LBP) caused by nucleus pulposus degeneration and calcification leads to great economic and social burden worldwide. Unexpectedly, no previous studies have demonstrated the association and the underlying mechanism between nucleus pulposus tissue degeneration and calcification formation. Secreted Phosphoprotein 1 (SPP1) exerts crucial functions in bone matrix mineralization and calcium deposition. Here, a novel function of SPP1 is reported, namely that it can aggravate nucleus pulposus cells (NPs) degeneration by negatively regulating extracellular matrix homeostasis. The degenerated NPs have a higher mineralization potential, which is achieved by SPP1. Mechanistically, SPP1 can accelerate the degeneration of nucleus pulposus cells by activating integrin α5β1 (ITGα5/β1), aggravating mitochondrial damage and inhibiting mitophagy. SPP1-ITGα5/β1 axis inhibits mitophagy by PINK1/PARKIN pathway blockade. In conclusion, SPP1 activates ITGα5/β1 to inhibit mitophagy, accelerates NPs degeneration, and induces calcification, thereby leading to intervertebral disc degeneration (IVDD) and calcification, identifying the potentially unknown mechanism and relationship between IVDD and calcification. Important insights are provided into the role of SPP1 in nucleus pulposus calcification in IVDD by inducing nucleus pulposus cell senescence through inhibition of mitophagy and may help develop potential new strategies for IVDD treatment.

Indexed as

CalcinosisIntegrin alpha5beta1Intervertebral Disc DegenerationMitophagyNucleus PulposusOsteopontinProtein KinasesUbiquitin-Protein LigasesAnimalsCells, CulturedHumansMalePTEN-Induced Putative KinaseRatsSignal TransductionUbiquitinIntegrin alpha5beta1Osteopontinparkin proteinProtein KinasesPTEN-Induced Putative KinaseUbiquitinUbiquitin-Protein Ligasescalcificationintegrin α5β1intervertebral disc degenerationmitophagynucleus pulposus cellssecreted phosphoprotein 1

Identifiers

PMID39721032
PMCPMC11831503

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.