Evidence map›Paper›PMID 39727652›Full record

ArticleDiseases (Basel, Switzerland)2024

Cyclosporine A Causes Gingival Overgrowth by Promoting Entry into the S Phase at the G1/S Cell Cycle Checkpoint in Gingival Fibroblasts Exposed to Lipopolysaccharide.

Reiri Takeuchi, Noriko Kuwahara, Yuta Amino, Sachiyo Hayashi, Chieko Taguchi, Itaru Suzuki, Haruka Suzuki, Teruaki Nagashima, Kazumune Arikawa, Yuichiro Okada and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diseases (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Reiri TakeuchiDepartment of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.ORCID 0000-0002-5122-3736
Noriko KuwaharaDepartment of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Yuta AminoDepartment of Oral Implantology, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Sachiyo HayashiDepartment of Special Needs Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Chieko TaguchiDepartment of Community Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Itaru SuzukiDepartment of Community Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Haruka SuzukiDepartment of Community Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Teruaki NagashimaDepartment of Community Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Kazumune ArikawaDepartment of Community Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Yuichiro OkadaDepartment of Histology, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Takato NomotoDepartment of Special Needs Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.
Koichi HiratsukaDepartment of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Matsudo 271-8587, Chiba, Japan.

Funding

JSPS KAKENHI 23K09514
6 · The paper itself

Abstract

objectivesCyclosporine A promotes gingival fibrosis by enhancing the proliferation of gingival fibroblasts, leading to gingival overgrowth. The population of gingival fibroblasts is regulated by cell cycle machinery, which balances cell growth and inhibition. Cells that detect DNA damage pause at the G1/S checkpoint to repair the damage instead of progressing to the S phase. Previous studies have linked drug-induced gingival overgrowth to the response of fibroblasts to lipopolysaccharide (LPS) and cyclosporine A. This research investigates the effects of cyclosporine A on the G1/S checkpoint and its mediators in LPS-treated gingival fibroblasts to clarify the mechanisms behind cyclosporine-A-induced gingival overgrowth.

methodsSemi-confluent human gingival fibroblasts were treated with LPS or cyclosporine A in DMEM. Cell proliferation was evaluated by counting the total number of cells. The distribution of the cell cycle phases was analyzed using flow cytometry. Additionally, the expression levels of mRNAs and proteins related to cell cycle regulators were quantified by reverse-transcription quantitative PCR and Western blotting, respectively.

resultsCyclosporine A treatment significantly enhanced cell proliferation and the G1-S cell cycle transition. It increased the mRNA levels of

conclusionsGingival overgrowth induced by cyclosporine A could be attributed to these alterations.

Indexed as

cell cyclecyclosporine AG1/S checkpointG1-S transitiongingival fibroblastgingival overgrowthS-phase entry

Identifiers

PMID39727652
PMCPMC11727098

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.