Evidence map›Paper›PMID 39730529›Full record

ArticleScientific reports2024

Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli.

Haoran Liu, Kang Xu, ShuQi Sun, Yinbiao Wan, Bojia Zhang, Yang Song, Chuanzhuang Guo, Songsen Sui, Ruiming Wang, Piwu Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Haoran Liu *State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Kang Xu *Dongxiao Bioengineering (Shandong) Co., Ltd, Jinan, 250300, Shandong, People's Republic of China.
ShuQi SunState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Yinbiao WanState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Bojia ZhangState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Yang SongState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Chuanzhuang GuoDongxiao Bioengineering (Shandong) Co., Ltd, Jinan, 250300, Shandong, People's Republic of China.
Songsen SuiDongxiao Bioengineering (Shandong) Co., Ltd, Jinan, 250300, Shandong, People's Republic of China.
Ruiming WangState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Piwu LiState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Junqing WangState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Zhenshang XuState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China. xuzhenshang@126.com.
Ting WangState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China. tingwang_97@hotmail.com.

Funding

Key innovation Project of Qilu University of Technology (Shandong Academy of Sciences) 2022JBZ01-06Natural Science oundation of Shandong Province ZR2022MC059The Basic Research of Pilot Project for the Integration of Science, Education and Industry, Qilu University of Technology, Shandong Academy of Sciences The Basic Research of Pilot Project for the Integration of Science, Education and Industry, Qilu University of Technology, Shandong Academy of SciencesThe Natural Science Foundation of Shandong Province for Youth ZR2020QC235The Science and Technology Support Plan for Young People in Colleges and Universities of Shandong Province 2020KJE005
6 · The paper itself

Abstract

D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector. The recombinant E. coli strain pET22b(+) was successfully constructed and expressed, providing an efficient whole-cell catalyst for converting inexpensive D-fructose into D-allulose. Subsequently, we optimized the induction and incubation conditions step by step using the single-factor method and used Lactobacillus plantarum(LAB) 217-8 to enhance the purity of D-allulose in the system. Ultimately, the BL21/pET22b(+)-E. coli strain achieved a conversion rate of up to 33.91% under optimal conditions, converting D-fructose to D-allulose. After purification, the purity of D-allulose reached 64.73%. Efficient production of D-allulose is a significant achievement, paving the way for future probiotic applications in its conversion.

Indexed as

Escherichia coliFermentationFructoseArthrobacterCarbohydrate EpimerasesLactiplantibacillus plantarumMetabolic EngineeringCarbohydrate EpimerasesFructosepsicoseArthrobacter globiformis M30D-alluloseD-allulose 3-epimerasesFermentation optimizationLactobacillus plantarum 217-8

Identifiers

PMID39730529
PMCPMC11681098

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.