Evidence mapPaperPMID 39736444Full record

ArticleMolecular metabolism2025

Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions.

Miranda Movahed, Ruy A Louzada, Manuel Blandino-Rosano

Abstract read
In one paragraph

Article in Molecular metabolism, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Miranda MovahedDepartment of Internal Medicine, Division of Endocrinology, Diabetes and Metabolism, Miller School of Medicine, University of Miami, Miami, FL, USA.
Ruy A LouzadaDepartment of Internal Medicine, Division of Endocrinology, Diabetes and Metabolism, Miller School of Medicine, University of Miami, Miami, FL, USA.
Manuel Blandino-RosanoDepartment of Internal Medicine, Division of Endocrinology, Diabetes and Metabolism, Miller School of Medicine, University of Miami, Miami, FL, USA. Electronic address: mblandino@med.miami.edu.

Funding

Dynorphin, a novel paracrine factor that regulates insulin secretionR01DK132095 · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · 2025 to 2025
$345k
NIDDK NIH HHS R01 DK132095
6 · The paper itself

Abstract

objectiveDynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic β-cells. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.

methodsWe used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets.

resultsThe prodynorphin gene is significantly upregulated in β-cells under hyperglycemic conditions. In vitro studies revealed that increased glucose concentrations correlate with increased dynorphin expression, indicating a critical interplay involving Ca

conclusionsThis study demonstrates that the opioid peptide prodynorphin is expressed in mouse and human β-cells. Prodynorphin levels are regulated in parallel with insulin in response to glucose, palmitate, and amino acids. Our findings elucidate the signaling pathways involved, with CamKII playing a key role in regulating prodynorphin levels in β-cells. Finally, our findings are the first to demonstrate active dynorphin secretion from mouse and human islets in response to glucose.

Indexed as

DynorphinsHyperglycemiaInsulin-Secreting CellsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2Cells, CulturedEnkephalinsGlucoseHumansInsulinIslets of LangerhansMaleMiceMice, Inbred C57BLProtein PrecursorsCalcium-Calmodulin-Dependent Protein Kinase Type 2DynorphinsEnkephalinsGlucoseInsulinpreproenkephalinProtein PrecursorsBeta-cellCamKIIDynorphinIslet

Identifiers

PMID39736444
PMCPMC11846442

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.