Evidence map›Paper›PMID 39737924›Full record

ArticleNature communications2024

Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70.

Marc A Morizono, Kelly L McGuire, Natalie I Birouty, Mark A Herzik

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Proteolytic control of mitochondrial protein translocases.Protein science : a publication of the Protein Society · 2026
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Marc A MorizonoDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.ORCID 0000-0003-2261-4452
Kelly L McGuireDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
Natalie I BiroutyDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
Mark A HerzikDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA. mherzik@ucsd.edu.ORCID 0000-0001-6653-6682

Funding

MOLECULAR BIOPHYSICS TRAINING PROGRAMT32GM008326 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KOMIVES, ELIZABETH A. · 1989 to 2020
$7.5M
ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
Towards an Atomistic Understanding of Mitochondrial Protein Biogenesis (Equipment Supplement)R35GM138206 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HERZIK, MARK ANTHONY · 2020 to 2024
$2.1M
NIGMS NIH HHS R01 GM129325NIGMS NIH HHS R35 GM138206NIGMS NIH HHS T32 GM008326U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35-GM138206U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) T32-GM008326
6 · The paper itself

Abstract

Maintenance of protein homeostasis is necessary for cell viability and depends on a complex network of chaperones and co-chaperones, including the heat-shock protein 70 (Hsp70) system. In human mitochondria, mitochondrial Hsp70 (mortalin) and the nucleotide exchange factor (GrpEL1) work synergistically to stabilize proteins, assemble protein complexes, and facilitate protein import. However, our understanding of the molecular mechanisms guiding these processes is hampered by limited structural information. To elucidate these mechanistic details, we used cryoEM to determine structures of full-length human mortalin-GrpEL1 complexes in previously unobserved states. Our structures and molecular dynamics simulations allow us to delineate specific roles for mortalin-GrpEL1 interfaces and to identify steps in GrpEL1-mediated nucleotide and substrate release by mortalin. Subsequent analyses reveal conserved mechanisms across bacteria and mammals and facilitate a complete understanding of sequential nucleotide and substrate release for the Hsp70 chaperone system.

Indexed as

Cryoelectron MicroscopyHSP70 Heat-Shock ProteinsMitochondriaMolecular Dynamics SimulationHumansMitochondrial ProteinsNucleotidesProtein BindingHSP70 Heat-Shock ProteinsMitochondrial ProteinsmortalinNucleotides

Identifiers

PMID39737924
PMCPMC11685456

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.