Evidence mapPaperPMID 39738466Full record

ArticleScientific reports2024

Integrated analyses of Mendelian randomization, eQTL, and single-cell transcriptome identify CCN3 as a potential biomarker in aortic dissection.

Haoyu Ran, Changying Li, Syed M Musa Rizvi, Ruiqin Zhou, Lingwen Kong, Sun Shuangling, Yue Shao, Kejia Wu, Changzhu Duan, Jun Luo and 3 more

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Haoyu RanDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Changying LiDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Syed M Musa RizviDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Ruiqin ZhouDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Lingwen KongDepartment of Cardiothoracic Surgery, Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing, China.
Sun ShuanglingDepartment of Biochemistry, Chongqing Medical and Pharmaceutical College, Chongqing, China.
Yue ShaoDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Kejia WuDepartment of Cell Biology and Genetics, Center for Molecular Medicine and Oncology Research, Chongqing Medical University, Chongqing, China.
Changzhu DuanDepartment of Cell Biology and Genetics, Center for Molecular Medicine and Oncology Research, Chongqing Medical University, Chongqing, China.
Jun LuoDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Haoming ShiDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Qingchen WuDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. wuqingchencqmu@126.com.
Cheng ZhangDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. zhangchengcqmu@126.com.

Funding

Fund of the First Affiliated Hospital of Chongqing Medical University NO. PYJJ2022-07Innovation Fund for Graduate Students of Chongqing Medical Universities CYYY-BSYJSCXXXM-202337National Natural Science Foundation of China 82270506Natural Science Foundation of Chongqing, China CSTB2022NSCQ-MSX0817Project of innovation team for Graduate Teaching CYYY-YJSJXCX-202318
6 · The paper itself

Abstract

Plasma secretory proteins are associated with various diseases, including aortic dissection (AD). However, current research on the correlation between AD and plasma protein levels is scarce or lacks specificity. This study aimed to explore plasma secretory proteins as potential biomarkers for AD. Through genome-wide association studies, expression quantitative trait locus (eQTL) analysis, and human plasma protein profiling, we identified DBNL, NPC2, SUMF2, and TFPI as high-risk genes and CCN3, PRKCSH, TEX264, and TGFBR3 as low-risk genes for AD. Further cell localization and differential expression analysis of these eight genes were conducted using single-cell data. We also examined their expression in three Gene Expression Omnibus datasets, measured their mRNA levels in AD versus normal tissues using qPCR, and assessed their protein levels in patients' blood versus healthy individuals using enzyme-linked immunosorbent assay. Our findings suggest that CCN3, consistently downregulated in both mRNA and plasma levels during AD, may have a protective role. Initial enrichment analyses of differentially expressed CCN3 cells suggested their involvement in focal adhesion, actin cytoskeleton regulation, and the PI3K-Akt signaling pathway.

Indexed as

Aortic DissectionBiomarkersGenome-Wide Association StudyMendelian Randomization AnalysisNephroblastoma Overexpressed ProteinQuantitative Trait LociTranscriptomeFemaleGene Expression ProfilingHumansMaleProteoglycansReceptors, Transforming Growth Factor betaSingle-Cell AnalysisbetaglycanBiomarkersCCN3 protein, humanNephroblastoma Overexpressed ProteinProteoglycansReceptors, Transforming Growth Factor betaAortic dissectionCCN3eQTLGWASPlasma secretory proteins

Identifiers

PMID39738466
PMCPMC11685893

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.