Evidence map›Paper›PMID 39742521›Full record

ArticleThe American journal of tropical medicine and hygiene2025

A Rapid On-Site Detection Method for Plasmodium falciparum Carried by Mosquitoes Using Disc Microfluidic Isothermal Amplification.

Ning Jiang, Jie Bai, Wenqi Shan, Qiuming Zhou, Haowei Dong, Hao Yuan, Wenbing Zhong, Xiangyu Li, Yajun Ma, Ping Li and 1 more

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Article in The American journal of tropical medicine and hygiene, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ning JiangDepartment of Pathogenic Biology, Basic Medical College, Naval Medical University, Shanghai, China.
Jie BaiDepartment of Pathogenic Biology, Basic Medical College, Naval Medical University, Shanghai, China.
Wenqi ShanDepartment of Naval Medicine, Naval Medical University, Shanghai, China.
Qiuming ZhouDepartment of Naval Medicine, Naval Medical University, Shanghai, China.
Haowei DongDepartment of Pathogenic Biology, Basic Medical College, Naval Medical University, Shanghai, China.
Hao YuanDepartment of Naval Medicine, Naval Medical University, Shanghai, China.
Wenbing ZhongHaikou Center for Disease Control and Prevention, Hainan, China.
Xiangyu LiDepartment of Pathogenic Biology, Basic Medical College, Naval Medical University, Shanghai, China.
Yajun MaDepartment of Naval Medicine, Naval Medical University, Shanghai, China.
Ping LiHaikou Center for Disease Control and Prevention, Hainan, China.
Heng PengDepartment of Pathogenic Biology, Basic Medical College, Naval Medical University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapidly identifying Anopheles-carrying malaria parasites is crucial for imported malaria prevention. However, suitable methods still lack quick detection in limited-resource situations. In this study, disc microfluidic isothermal amplification integrating loop-mediated isothermal amplification (LAMP) and microfluidic chip technology were applied to develop rapid and precise detection with low resource requirements. Primer set EMP1G2, which is specific to Plasmodium falciparum (P. falciparum) erythrocyte membrane protein 1, and primer set 18sG2, which is specific to ribosomal 18s subunit RNA, were screened for optimal LAMP-specific primer sets. The minimum detection limits were 125 copies/µL for the EMP1G2 and 6,562 copies/µL for the 18sG2. Subsequently, optimal primer sets were evaluated for specificity with nucleic acid from other mosquito-borne pathogens and arthropod vectors. No nonspecific amplification was observed in optimal amplification-specific primer sets with the DNA of Anopheles mosquitoes and morphologically similar arthropods or with the copy DNA of Zika virus, yellow fever virus, or dengue virus 1. The detection method was evaluated in a simulated scenario and demonstrated a robust capacity for rapid on-site detection. Additionally, polymerase chain reaction (PCR) and quantitative real-time PCR methods were compared using this method. In this study, a rapid detection method based on disc microfluidic isothermal amplification was developed that could be used to detect P. falciparum carried by mosquitoes in a field setting under limited resource conditions.

Indexed as

AnophelesMalaria, FalciparumMicrofluidicsMolecular Diagnostic TechniquesMosquito VectorsNucleic Acid Amplification TechniquesPlasmodium falciparumAnimalsDNA PrimersHumansSensitivity and SpecificityDNA Primers

Identifiers

PMID39742521
PMCPMC11965755

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.