Evidence map›Paper›PMID 39745593›Full record

ArticleJournal of applied genetics2025

A 280 bp SINE insertion within the pig PLA2G16 could potentially modify gene expression through integration with its transcript.

Cai Chen, Mengli Wang, Yao Zheng, Ziyan Liu, Phiri Azele, Ahmed A Saleh, Xiaoyan Wang, Chengyi Song

Abstract read
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In one paragraph

Article in Journal of applied genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Cai ChenCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.ORCID http://orcid.org/0000-0001-6789-6006
Mengli WangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.ORCID http://orcid.org/0009-0007-6654-7577
Yao ZhengCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.ORCID http://orcid.org/0009-0007-2747-0298
Ziyan LiuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.ORCID http://orcid.org/0000-0002-4567-0521
Phiri AzeleAnimal Production, Zambia Institute of Animal Health, Ministry of Fisheries and Livestock, 670237, Mazabuka, Zambia.ORCID http://orcid.org/0009-0009-0555-8322
Ahmed A SalehCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.ORCID http://orcid.org/0000-0003-1314-8066
Xiaoyan WangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.ORCID http://orcid.org/0000-0001-8521-2974
Chengyi SongCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China. cysong@yzu.edu.cn.ORCID http://orcid.org/0000-0002-0488-4718

Funding

Graduate Research and Innovation Projects of Jiangsu Province JBGS(2021)028)the Open Project Program of International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement IJRLD-KF202209
6 · The paper itself

Abstract

In our previous study, we identified a Short Interspersed Nuclear Element Retrotransposon Insertion Polymorphism (SINE-RIP) within the 3' untranslated region (3'UTR) of the Phospholipase A2 Group XVI (PLA2G16) gene, which is essential in lipid metabolism. In this study, we confirmed the presence of this 280 bp SINE insertion and examined its distribution across ten distinct pig breeds using PCR and sequencing. Subsequently, RT-PCR was employed to determine its potential for co-transcription. Finally, qPCR analysis was performed to evaluate the insertion's effect on PLA2G16 expression. The results indicated significant polymorphism at this site among different breeds. The SINE insertion can co-transcribe with PLA2G16 and shows a tissue-specific relationship with its expression in backfat and liver. Specifically, in Sujiang and Mi pigs, individuals homozygous for the SINE insertion (SINE

Indexed as

Mutagenesis, InsertionalShort Interspersed Nucleotide Elements3' Untranslated RegionsAnimalsLiverPolymorphism, GeneticSwine3' Untranslated RegionsSINE; 3′UTR; Fusion transcripts; PLA2G16

Identifiers

PMID39745593

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.