Evidence map›Paper›PMID 39747977›Full record

ArticleScientific reports2025

Clinical impacts of Artocarpus lakoocha agglutinin-binding glycans for prognosis and treatment of cholangiocarcinoma.

Phisit Sintusen, Kulthida Vaeteewoottacharn, Ubon Cha'on, Chawalit Pairojkul, Chaiwat Aphivatanasiri, Sutas Suttiprapa, Panupong Mahalapbutr, Atit Silsirivanit, Sopit Wongkham, Sukanya Luang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Phisit SintusenDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Kulthida VaeteewoottacharnDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Ubon Cha'onDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chawalit PairojkulDepartment of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chaiwat AphivatanasiriDepartment of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Sutas SuttiprapaDepartment of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Panupong MahalapbutrDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Atit SilsirivanitDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Sopit WongkhamDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Sukanya LuangDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. sukany@kku.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Artocarpus lakoocha agglutinin (ALA), which specifically targets the Gal/GalNAc components of complex glycans, was isolated from the seeds of Artocarpus lakoocha. This study is the first to explore the role of ALA in identifying aberrant glycans, designated ALA-binding glycans (ALAG), and its implications in cholangiocarcinoma (CCA). ALA-histochemistry was used to evaluate ALAG expression in liver fluke-induced CCA tissues from hamsters (n = 60). Elevated ALAG expression was observed in hyperplastic ducts and significantly increased in CCA tissues, while normal biliary epithelium and hepatocytes showed no expression. Similar results were found in patient CCA tissues (n = 68), where higher ALAG levels correlated with shorter survival rates, indicating the involvement of ALAG in CCA development and progression. Furthermore, ALA treatment inhibited cell viability in CCA cell lines, as demonstrated by MTT and colony formation assays, and Ki-67 expression. ALA treatment also decreased cell migration and invasion, as shown by Transwell assays. Gelatin zymography suggested that these effects might be associated with reduced MMP-9 activity. Overall, these findings may position ALAG as a potential marker for poor prognosis in CCA, while ALA may serve as a novel lectin for both detection and therapeutic applications in CCA.

Indexed as

ArtocarpusBile Duct NeoplasmsCholangiocarcinomaPolysaccharidesAnimalsCell Line, TumorCell MovementCell ProliferationCell SurvivalCricetinaeFemaleHumansMaleMatrix Metalloproteinase 9Middle AgedPlant LectinsMatrix Metalloproteinase 9Plant LectinsPolysaccharidesAberrant glycosylationAnti-metastasisAnti-proliferationArtocarpus lakoocha agglutininCholangiocarcinomaT and Tn antigens

Identifiers

PMID39747977
PMCPMC11696817

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.