Evidence map›Paper›PMID 39748076›Full record

ArticleScientific reports2025

Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli.

Tianzhen Xiong, Xinmeng Li, Wei Liu, Huidie Yue, Junling Liu, Dingyuan Bai, Wei Li, Guangyan Fan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Systems metabolic engineering for hydroxytyrosol production inApplied and environmental microbiology · 2026
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tianzhen XiongDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China. tzxiong@xynu.edu.cn.
Xinmeng LiDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.
Wei LiuInstitute of Agricultural Quality Standards and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun, 130000, Jilin, China.
Huidie YueDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.
Junling LiuDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.
Dingyuan BaiDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.
Wei LiDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.
Guangyan FanDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.

Funding

Key scientific research project of higher education institutions in Henan Province 24B180017Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX19_1841
6 · The paper itself

Abstract

Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (yahK) and glucose dehydrogenase (gdh). We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production. The yield of hydroxytyrosol was further increased 89.3% by optimizing the temperature and pH. Finally, 55.3 mM hydroxytyrosol was produced within 14 h by fed-batch biotransformation. This study provides a novel approach for hydroxytyrosol production.

Indexed as

Escherichia coliMetabolic EngineeringPhenylethyl AlcoholGlucose 1-DehydrogenaseHydrogen-Ion ConcentrationPlasmidsTemperatureTyrosine Phenol-Lyase3,4-dihydroxyphenylethanolGlucose 1-DehydrogenasePhenylethyl AlcoholTyrosine Phenol-LyaseCascade reactionEscherichia coliHydroxytyrosolWhole cell biotransformation

Identifiers

PMID39748076
PMCPMC11696566

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.