Evidence map›Paper›PMID 39754637›Full record

ArticleFood and environmental virology2025

Detection of Rocahepevirus ratti in Bivalve Mollusks from São Luís Island, Maranhão, Brazil: A Potential Transmission Route of an Emerging Zoonotic Pathogen?

Andreza Soriano Figueiredo, Isabella Rodrigues Negreiros, Aldaleia do Nascimento E Silva, Caroline Roberta Soares Salgado, Natália Lourenço Dos Santos, Marcelo Alves Pinto, Alcina Vieira de Carvalho Neta, José Paulo Gagliardi Leite, Carina Pacheco Cantelli

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In one paragraph

Article in Food and environmental virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Rat Hepatitis E Virus (Foods (Basel, Switzerland) · 2025
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Andreza Soriano FigueiredoLaboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil. andrezasofi@gmail.com.
Isabella Rodrigues NegreirosProgram in Animal Science, State of Maranhão University, São Luís, Maranhão, Brazil.
Aldaleia do Nascimento E SilvaLaboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
Caroline Roberta Soares SalgadoLaboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
Natália Lourenço Dos SantosLaboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
Marcelo Alves PintoLaboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
Alcina Vieira de Carvalho NetaProgram in Animal Science, State of Maranhão University, São Luís, Maranhão, Brazil.
José Paulo Gagliardi LeiteLaboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
Carina Pacheco CantelliLaboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.

Funding

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro FAPERJ E-26/210.045/2023Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro FAPERJ/SEI-260003/001753/2023The Brazilian National Council for Scientific and Technological Development (CNPq) 402639/2023-5The Brazilian National Council for Scientific and Technological Development (CNPq) 402987/2023-3
6 · The paper itself

Abstract

The attempt to investigate hepatitis E virus (HEV) contamination in naturally growing mangrove bivalve mollusks captured for local sale in a touristic area of Maranhão state in Brazil revealed the detection of rat hepatitis E virus (ratHEV). Using international standard protocols for processing and nucleic acid extraction, we analyzed 89 bivalve samples (Mytella falcata and Crassostrea rhizophorae) with two broadly reactive assays: heminested pan-Hepeviridae (ORF-1) and probe-based HEV-1 to HEV-4 (ORF-2/ORF-3). Heminested reactions presented 2 (2.2%) amplifications of the expected size. Nucleotide identities ranged from 86.6 to 89.0% with ratHEV isolates from wild rats, pigs, one human case and sewage reported in different countries. Regarding the phylogenetic tree, the sequences grouped with genotype HEV-C1. This first report of ratHEV detection in bivalve mollusks may be a starting point for further research on virus variability, distribution, host range and especially the possible role of contaminated shellfish as a vehicle for ratHEV transmission.

Indexed as

BivalviaPhylogenyShellfishAnimalsBrazilHepatitis E virusHumansIslandsRatsBivalve molluskFood safetyHepatitis E virusHEV-C1Rocahepevirus rattiZoonosis

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.