Evidence mapPaperPMID 39756490Full record

ArticleFree radical biology & medicine2025

E-cigarette-induced changes in cell stress and mitochondrial function.

Ramamurthy Chitteti, Juan Pablo Zuniga-Hertz, Jorge A Masso-Silva, John Shin, Ingrid Niesman, Christine M Bojanowski, Avnee J Kumar, Mark Hepokoski, Laura E Crotty Alexander, Hemal H Patel and 1 more

Abstract read
In one paragraph

Article in Free radical biology & medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ramamurthy ChittetiVA San Diego Healthcare System, San Diego, CA, USA; Department of Anesthesiology, School of Medicine, University of California San Diego, USA. Electronic address: rchitteti@health.ucsd.edu.
Juan Pablo Zuniga-HertzVA San Diego Healthcare System, San Diego, CA, USA; Department of Anesthesiology, School of Medicine, University of California San Diego, USA.
Jorge A Masso-SilvaVA San Diego Healthcare System, San Diego, CA, USA; Division of Pulmonary, Critical Care and Sleep Medicine, University of California San Diego, USA.
John ShinDivision of Pulmonary, Critical Care and Sleep Medicine, University of California San Diego, USA.
Ingrid NiesmanSan Diego State University, Electron Microscope Facility, 5500 Campanile Dr, San Diego, CA, 92182, USA.
Christine M BojanowskiDivision of Pulmonary, Critical Care and Sleep Medicine, University of California San Diego, USA; Division of Pulmonary and Critical Care, Tulane University, New Orleans, LA, USA.
Avnee J KumarVA San Diego Healthcare System, San Diego, CA, USA; Division of Pulmonary, Critical Care and Sleep Medicine, University of California San Diego, USA.
Mark HepokoskiVA San Diego Healthcare System, San Diego, CA, USA; Division of Pulmonary, Critical Care and Sleep Medicine, University of California San Diego, USA.
Laura E Crotty AlexanderVA San Diego Healthcare System, San Diego, CA, USA; Division of Pulmonary, Critical Care and Sleep Medicine, University of California San Diego, USA.
Hemal H PatelVA San Diego Healthcare System, San Diego, CA, USA; Department of Anesthesiology, School of Medicine, University of California San Diego, USA.
David M RothVA San Diego Healthcare System, San Diego, CA, USA; Department of Anesthesiology, School of Medicine, University of California San Diego, USA.

Funding

Developing a Diverse Next Generation of Leaders in Respiratory ScienceT32HL166127 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2023 to 2025
$761k
Convergence of Vitamin E, THC, Nicotine, Propylene Glycol and Glycerin Effects on Lung Inflammation When VapedK24HL155884 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2023 to 2025
$365k
BLRD VA I01 BX001963BLRD VA I01 BX004767BLRD VA IK6 BX005229NCIRD CDC HHS U01 IP000040NCIRD CDC HHS U01 IP000965NHLBI NIH HHS K24 HL155884NHLBI NIH HHS R01 HL137052NHLBI NIH HHS T32 HL166127NIA NIH HHS R21 AG058174
6 · The paper itself

Abstract

Inhaling aerosols from electronic nicotine delivery systems, such as e-cigarettes (e-cigs), may pose health risks beyond those caused by nicotine intake. Exposure to e-cig aerosols can lead to the release of exosomes and metabolites into the bloodstream, potentially affecting mitochondrial physiology across the body, leading to chronic inflammatory diseases. In this study we assessed the effects of e-cig use by young healthy human subjects on the circulating exosome profile and markers of cell stress, and also defined the effects of e-cig user plasma on mitochondrial function in endothelial cells (EA. Hy 926) and epithelial cells (A549) via adoptive transfer. E-cig users had altered plasma exosome profiles, with significantly increased levels of cell free mitochondrial DNA (mtDNA), protein carbonyls, and 4-HNE relative to non-users. Plasma from e-cig users decreased maximal mitochondrial respiration and spare capacity of cells, while also increasing metabolic stress, as evidenced by changes in mitochondrial phenotype from basal to stressed in both endothelial and epithelial cells, which was corroborated by electron microscopy demonstrating structural changes in mitochondria. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) levels significantly increased in e-cig plasma-subjected cells. Overall, we identified alterations in plasma exosome profiles and increased markers of mitochondrial stress in e-cig users and evidence that circulating factors within plasma from e-cig users drives metabolic stress in endothelial and epithelial cells. Our results imply that e-cig use adversely affects mitochondrial function, leading to stress and potentially chronic inflammation across the body.

Indexed as

Electronic Nicotine Delivery SystemsMitochondriaAdultDNA, MitochondrialEndothelial CellsEpithelial CellsExosomesFemaleHumansMaleMembrane Potential, MitochondrialNicotineOxidative StressReactive Oxygen SpeciesVapingYoung AdultDNA, MitochondrialNicotineReactive Oxygen SpeciesElectronic (e)-cigaretteEndothelial cellsEpithelial cellsMetabolic stressMitochondriaReactive oxygen speciesTobacco

Identifiers

PMID39756490
PMCPMC12834502

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.