Evidence map›Paper›PMID 39757032›Full record

ArticleInternational dental journal2025

Screening Differentially Expressed Proteins in Areca Nut-Related Oral Squamous Cell Carcinoma Using Tandem Mass Tag Proteomics.

Xiaoqun Mai, Xinyu Chen, Zihan Wang, Haiyu Xian, Qitao Wen, Guanyu Sun, Tao Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International dental journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaoqun MaiDepartment of stomatology, Hainan General Hospital, Haikou, China; The Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan, China.
Xinyu ChenCollege & Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China. Electronic address: chenxinyu814@sr.gxmu.edu.cn.
Zihan WangSchool of Stomatology, Shanxi Medical University, Taiyuan, Shanxi, China.
Haiyu XianDepartment of stomatology, Hainan General Hospital, Haikou, China; The Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan, China.
Qitao WenDepartment of stomatology, Hainan General Hospital, Haikou, China; The Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan, China.
Guanyu SunDepartment of stomatology, Hainan General Hospital, Haikou, China; The Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan, China.
Tao WangDepartment of stomatology, Hainan General Hospital, Haikou, China; The Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan, China. Electronic address: 18608917377@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAreca nut chewing has been associated with a poor prognosis in oral squamous cell carcinoma (OSCC). This study seeks to identify differentially expressed proteins among areca nut-related OSCC,non-areca nut-related OSCC and adjacent normal epithelial tissues, with the aim of providing novel insights for the investigation of areca nut-related OSCC.

methodsUsing Tandem mass tag (TMT)-based proteomic analysis, a comparative proteomic profiling was conducted among areca nut-related OSCC, non-areca nut-related OSCC, and adjacent normal epithelial tissues (n=15). GO and KEGG enrichment analyses were then employed to identify significant proteins pertinent to the pathogenesis of OSCC for further study. Western Blot (WB) and Immunohistochemistry (IHC) techniques were used to preliminary validate the expression patterns of LZTS1, MMP10, MYH6, MB, and TNNC1 among various groups (n=30).

results27 differentially expressed proteins were identified when comparing the areca nut-related OSCC group with both the non-areca nut-related OSCC and normal epithelial tissue groups. Among these, 15 proteins were upregulated, while 12 were downregulated. LZTS1 and MMP10 were included in the upregulated proteins, whereas MYH6, MB, and TNNC1 were downregulated. WB and IHC analyses corroborated the proteomic findings, revealing consistent expression trends for these 5 proteins across the studied groups.

conclusionLZTS1, MMP10, MYH6, MB and TNNC1 emerge as promising biomarkers for assessing disease progression, prognosis, and potential targeted therapies in areca nut-related OSCC.

Indexed as

ArecaCarcinoma, Squamous CellMouth NeoplasmsNutsProteomicsBiomarkers, TumorBlotting, WesternFemaleHumansImmunohistochemistryMaleMiddle AgedTandem Mass SpectrometryBiomarkers, TumorAreca nutBiomarkersOral squamous cell carcinoma (OSCC)ProteomicsTandem mass tag (TMT)

Identifiers

PMID39757032
PMCPMC12142794

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.