Evidence map›Paper›PMID 39757463›Full record

ArticleAdvanced healthcare materials2025

In Vitro Engineered ECM-incorporated Hydrogels for Osteochondral Tissue Repair: A Cell-Free Approach.

Ali Coyle, Aishik Chakraborty, Jiaqi Huang, Yasmeen Shamiya, Wei Luo, Arghya Paul

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ali CoyleSchool of Biomedical Engineering, The University of Western Ontario, London, ON, N6A 5B9, Canada.
Aishik ChakrabortyDepartment of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON, N6A 5B9, Canada.
Jiaqi HuangDepartment of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON, N6A 5B9, Canada.
Yasmeen ShamiyaDepartment of Chemistry, The University of Western Ontario, London, ON, N6A 5B9, Canada.
Wei LuoSchool of Biomedical Engineering, The University of Western Ontario, London, ON, N6A 5B9, Canada.
Arghya PaulSchool of Biomedical Engineering, The University of Western Ontario, London, ON, N6A 5B9, Canada.ORCID 0000-0003-4788-0378

Funding

Natural Sciences and Engineering Research Council of Canada CRC-2018-00028
6 · The paper itself

Abstract

Prevalence of osteoarthritis has been increasing in aging populations, which has necessitated the use of advanced biomedical treatments. These involve grafts or delivering drug molecules entrapped in scaffolds. However, such treatments often show suboptimal therapeutic effects due to poor half-life and off-target effects of drug molecules. As a countermeasure, a 3D printable robust hydrogel-based tissue-repair platform is developed containing decellularized extracellular matrix (dECM) from differentiated mammalian cells as the therapeutic cargo. Here, pre-osteoblastic and pre-chondrogenic murine cells are differentiated in vitro, decellularized, and incorporated into methacrylated gelatin (GelMA) solutions to form osteogenic (GelO) and chondrogenic (GelC) hydrogels, respectively. Integrating the bioactive dECM from differentiated cell sources allows GelO and GelC to induce differentiation in human adipose-derived stem cells (hASCs) toward osteogenic and chondrogenic lineages. Further, GelO and GelC can be covalently adhered using a carbodiimide coupling reaction, forming a multi-layered hydrogel with potential application as a bioactive osteochondral plug. The designed multi-layered hydrogel can also induce differentiation of hASCs in vitro. In conclusion, the bioactive dECM carrying 3D printed robust hydrogel offers a promising new drug and cell-free therapeutic strategy for bone and cartilage repair and future osteoarthritis management.

Indexed as

ChondrogenesisDecellularized Extracellular MatrixExtracellular MatrixHydrogelsOsteogenesisTissue EngineeringAnimalsCell DifferentiationHumansMiceStem CellsTissue ScaffoldsDecellularized Extracellular MatrixHydrogels3D printingbone regenerationcartilage regenerationcell‐derived extracellular matrix (ECM)mesenchymal stem cells

Identifiers

PMID39757463
PMCPMC11804842

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.