Evidence mapPaperPMID 39757707Full record

ArticleCancer medicine2025

Molecular Mechanisms of Synergistic Effect of PRIMA-1

Xiao-Lan Li, Jianbiao Zhou, Nicole Xin-Ning Tang, Yi Chai, Meng Zhou, Ai-di Gao, Zhong-Kai Lu, Han Min

Abstract read
In one paragraph

Article in Cancer medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiao-Lan LiDepartment of Gastroenterology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, People's Republic of China.ORCID https://orcid.org/0000-0001-6678-5494
Jianbiao ZhouCancer Science Institute of Singapore, National University of Singapore, Singapore.
Nicole Xin-Ning TangCancer Science Institute of Singapore, National University of Singapore, Singapore.
Yi ChaiDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Meng ZhouChangzhou No. 4 People's Hospital, Changzhou City, Jiangsu Province, People's Republic of China.
Ai-di GaoDepartment of Gastroenterology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, People's Republic of China.
Zhong-Kai LuDepartment of Gastroenterology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, People's Republic of China.
Han MinDepartment of Gastroenterology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, People's Republic of China.ORCID https://orcid.org/0009-0004-3115-1247

Funding

National Natural Science Foundation of China 81603128Suzhou Municipal Science and Technology Bureau SKY2023210
6 · The paper itself

Abstract

backgroundThe toxicity and drug resistance associated with oxaliplatin (L-OHP) limit its long-term use for colorectal cancer (CRC) patients. p53 mutation is a common genetic trait of CRC. PRIMA-1

methodsCell viability was assessed with Cell Counting Kit-8 (CCK-8) assay and combination index (CI) was calculated using The Chou-Talalay method. We also employed wound healing assay and colony formation assay to determine the effect of L-OHP, PRIMA-1

resultsOur findings showed heightened cytotoxicity and inhibition of migration, and colony formation in CRC cells treated with both drugs, irrespective of p53 status, presenting a promising avenue for addressing L-OHP resistance and toxicity. RNA-seq analysis revealed differential responses between p53-wide type HCT116 and p53-mutant DLD-1 cells, with pathway alterations implicated in tumorigenesis. WGCNA identified key modules and hub genes associated with combination therapy response. In vivo studies demonstrated enhanced efficacy of combined therapy over PRIMA-1

conclusionsIn summary, our research reveals the differential molecular mechanisms of combined PRIMA-1

Indexed as

Antineoplastic Combined Chemotherapy ProtocolsColorectal NeoplasmsDrug SynergismOxaliplatinTumor Suppressor Protein p53Xenograft Model Antitumor AssaysAnimalsCell Line, TumorCell ProliferationCell SurvivalFemaleGene Expression Regulation, NeoplasticHCT116 CellsHumansMiceMice, NudeeprenetapoptOxaliplatinQuinuclidinesTP53 protein, humanTumor Suppressor Protein p53colorectal cancer (CRC)combination therapydrug resistancehematologic toxicityoxaliplatin (L‐OHP)p53 tumor suppressor genePRIMA‐1met

Identifiers

PMID39757707
PMCPMC11702439

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.