Evidence map›Paper›PMID 39763840›Full record

ArticlebioRxiv : the preprint server for biology2025

Ultrastructural membrane dynamics of mouse and human cortical synapses.

Chelsy R Eddings, Minghua Fan, Yuuta Imoto, Kie Itoh, Xiomara McDonald, Jens Eilers, William S Anderson, Paul F Worley, Kristina Lippmann, David W Nauen and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Chelsy R EddingsDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.ORCID 0000-0003-3003-8379
Minghua FanSolomon H. Snyder Department of Neuroscience, The Johns Hopkins University, Baltimore MD, 21205, USA.
Yuuta ImotoDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.
Kie ItohDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.
Xiomara McDonaldDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.
Jens EilersCarl-Ludwig-Institute of Physiology, Faculty of Medicine, Leipzig University, Leipzig 04103, Germany.
William S AndersonDepartment of Neurosurgery, The Johns Hopkins Hospital, Baltimore, MD, 21205, USA.
Paul F WorleySolomon H. Snyder Department of Neuroscience, The Johns Hopkins University, Baltimore MD, 21205, USA.
Kristina LippmannCarl-Ludwig-Institute of Physiology, Faculty of Medicine, Leipzig University, Leipzig 04103, Germany.
David W NauenDepartment of Neurology, The Johns Hopkins Hospital, Baltimore, Maryland, 21205, USA.
Shigeki WatanabeDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.ORCID 0000-0001-7580-8141

Funding

Research Education ComponentP30AG066507 · NIA · JOHNS HOPKINS UNIVERSITY · PI JUAN TRONCOSO · 2020 to 2026
$29.3M
NPTX2: Preserving memory circuits in normative aging and Alzheimer's DiseaseR01AG072643 · NIA · UNIVERSITY OF ARIZONA · PI BARNES, CAROL A. · 2021 to 2025
$5.7M
Ultrafast membrane trafficking at synapsesR35NS132153 · NINDS · JOHNS HOPKINS UNIVERSITY · PI Shigeki Watanabe · 2023 to 2026
$4.1M
Reviving electron microscopy for synaptic cell biologyDP2NS111133 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2018
$2.5M
Spatial and molecular determinants of fusion probability and timingR01NS105810 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2022
$1.8M
Request for Leica High-pressure Freezer and Automated Freeze-substitution SystemS10RR026445 · NCRR · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2010 to 2010
$273k
NCRR NIH HHS S10 RR026445NIA NIH HHS P30 AG066507NIA NIH HHS R01 AG072643NINDS NIH HHS DP2 NS111133NINDS NIH HHS R01 NS105810NINDS NIH HHS R35 NS132153
6 · The paper itself

Abstract

Live human brain tissues provide unique opportunities for understanding synaptic transmission. Investigations have been limited to anatomy, electrophysiology, and protein localization-while crucial parameters such as synaptic vesicle dynamics were not visualized. Here we utilize zap-and-freeze time-resolved electron microscopy to overcome this hurdle. First, we validate the approach with acute mouse brain slices to demonstrate that slices can be stimulated to produce calcium signaling. Next, we show that synaptic vesicle endocytosis is induced in both mouse and human brain slices. Crucially, clathrin-free endocytic pits appear immediately next to the active zone, where ultrafast endocytosis normally occurs, and can be trapped at this location by a dynamin inhibitor. In both species a protein essential for ultrafast endocytosis, Dynamin 1xA, localizes to the region peripheral to the active zone, the putative endocytic zone, indicating a possible conserved mechanism between mouse and human. This approach has the potential to reveal dynamic, high-resolution information about synaptic membrane trafficking in intact human brain slices.

Indexed as

2-photon calcium imagingcerebellumcortexDynamin 1xAhigh-pressure freezinghuman neocortexstimulated emission depletion microscopysynaptic transmissionsynaptic vesicle endocytosistime-resolved electron microscopyultrafast endocytosiszap-and-freeze

Identifiers

PMID39763840
PMCPMC11703259

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.