Evidence map›Paper›PMID 39769183›Full record

ArticleInternational journal of molecular sciences2024

Functional and Biological Characterization of the LGR5Δ5 Splice Variant in HEK293T Cells.

Matthias Kappler, Laura Thielemann, Markus Glaß, Laura Caggegi, Antje Güttler, Jonas Pyko, Sarah Blauschmidt, Tony Gutschner, Helge Taubert, Sven Otto and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Matthias KapplerDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.ORCID 0000-0003-2002-0271
Laura ThielemannDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Markus GlaßInstitute of Molecular Medicine, Section for Molecular Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.ORCID 0000-0003-2718-8907
Laura CaggegiDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Antje GüttlerDepartment of Radiotherapy, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.ORCID 0000-0003-3214-8694
Jonas PykoInstitute of Molecular Medicine, Section for RNA Biology and Pathogenesis, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.ORCID 0009-0003-0589-5157
Sarah BlauschmidtDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Tony GutschnerInstitute of Molecular Medicine, Section for RNA Biology and Pathogenesis, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.ORCID 0000-0002-1722-3134
Helge TaubertDepartment of Urology, University Hospital Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, 91054 Erlangen, Germany.ORCID 0000-0002-9077-1727
Sven OttoDepartment of Oral and Maxillofacial Surgery and Facial Plastic Surgery, Ludwig Maximilians University, 80337 Munich, Germany.
Alexander W EckertDepartment of Cranio Maxillofacial Surgery, Paracelsus Medical University, 90471 Nuremberg, Germany.
Frank TavassolDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Matthias BacheDepartment of Radiotherapy, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Dirk VordermarkDepartment of Radiotherapy, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Tom KauneDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
Swetlana RotDepartment of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.

Funding

Dr. Kleist Stiftung (Foundation) NAOpen Access Publication Fund of the Martin Luther University Halle-Wittenberg NA
6 · The paper itself

Abstract

The regulator of the canonical Wnt pathway, leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), is expressed in the stem cell compartment of several tissues and overexpressed in different human carcinomas. The isoform of the stem cell marker LGR5, named LGR5Δ5 and first described by our group, is associated with prognosis and metastasis in oral squamous cell carcinoma (OSCC) and soft tissue sarcoma (STS). In a proof-of-principle analysis, the function of LGR5Δ5 was investigated in HEK293T cells, a model cell line of the Wnt pathway, compared to full-length LGR5 (FL) expression. The CRISPR/CAS knockout of

Indexed as

Receptors, G-Protein-CoupledThrombospondinsCell MovementCRISPR-Cas SystemsHEK293 CellsHumansProtein IsoformsR-SpondinsWnt Signaling PathwayLGR4 protein, humanLGR5 protein, humanProtein IsoformsReceptors, G-Protein-CoupledRSPO1 protein, humanR-SpondinsThrombospondinsCRISPR/CASisoformLGR4LGR5LGR5Δ5stem cell marker

Identifiers

PMID39769183
PMCPMC11678308

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.