Evidence mapPaperPMID 39769358Full record

ArticleInternational journal of molecular sciences2024

SNPs in GPCR Genes and Impaired Osteogenic Potency in Osteoporotic Patient Lines-Based Study.

Julia Sopova, Olga Krasnova, Giomar Vasilieva, Anna Zhuk, Olga Lesnyak, Vitaliy Karelkin, Irina Neganova

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Observational
  3. Review
  4. Novel SNP Combination for Predictive Osteoporotic Diagnosis.International journal of molecular sciences · 2025
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Julia SopovaInstitute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia.ORCID 0000-0002-7825-273X
Olga KrasnovaInstitute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia.
Giomar VasilievaInstitute of Applied Computer Science, ITMO University, St. Petersburg 197101, Russia.
Anna ZhukLaboratory of Amyloid Biology, Saint-Petersburg State University, St. Petersburg 199034, Russia.ORCID 0000-0001-8683-9533
Olga LesnyakDepartment of Family Medicine, North-Western State Medical University named after I.I. Mechnikov, St. Petersburg 195298, Russia.ORCID 0000-0002-0143-0614
Vitaliy KarelkinVreden National Medical Research Center of Traumatology and Orthopedics, St. Petersburg 195427, Russia.
Irina NeganovaInstitute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia.

Funding

Ministry of Science and Higher Education of the Russian Federation Agreement No. 075-15-2021-1075 dated 09/28/2021
6 · The paper itself

Abstract

G-protein-coupled receptors (GPCRs) have emerged as critical regulators of bone development and remodeling. In this study, we aimed to identify specific GPCR mutations in osteoporotic patients via next-generation sequencing (NGS). We performed NGS sequencing of six genomic DNA samples taken from osteoporotic patients and two genomic DNA samples from healthy donors. Next, we searched for single-nucleotide polymorphisms (SNPs) in GPCR genes that are associated with osteoporosis. For three osteoporotic patients and one healthy donor, bone biopsies were used to generate patient-specific mesenchymal stem cell (MSC) lines, and their ability to undergo osteodifferentiation was analyzed. We found that MSCs derived from osteoporotic patients have a different response to osteoinductive factors and impaired osteogenic differentiation using qPCR and histochemical staining assays. The NGS analysis revealed specific combinations of SNPs in GPCR genes in these patients, where SNPs in

Indexed as

Cell DifferentiationMesenchymal Stem CellsOsteogenesisOsteoporosisPolymorphism, Single NucleotideReceptors, G-Protein-CoupledAgedCell LineFemaleHigh-Throughput Nucleotide SequencingHumansMaleMiddle AgedMutationReceptors, G-Protein-CoupledGPCRnext-generation sequencingosteodifferentiationosteoporosispatient-specific stem cells

Identifiers

PMID39769358
PMCPMC11677449

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.