Evidence map›Paper›PMID 39770294›Full record

ArticlePathogens (Basel, Switzerland)2024

Development, Optimization, and Validation of a Quantitative PCR Assay for

Julie Lewis, Vett K Lloyd, Gilles A Robichaud

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Julie LewisDepartment of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada.
Vett K LloydDepartment of Biology, Mount Allison University, Sackville, NB E4L 1G7, Canada.
Gilles A RobichaudDepartment of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada.ORCID 0000-0002-5782-7899

Funding

Bourse d'études et de recherche de Université de Moncton 2018Canadian Lyme Disease Foundation 2018Mount Allison Lyme Disease Research Fund 2018Natural Sciences and Engineering Research Council 2015
6 · The paper itself

Abstract

Tick-borne pathogens are growing in importance for human and veterinary research worldwide. We developed, optimized, and validated a reliable quantitative PCR (qPCR; real-time PCR) assay to assess Borrelia burgdorferi infection by targeting two B. burgdorferi genes,

Indexed as

Animals, WildBorrelia burgdorferiLyme DiseaseReal-Time Polymerase Chain ReactionSensitivity and SpecificityTicksAnimalsAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesFlagellinHumansLipoproteinsAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesflaB flagellinFlagellinLipoproteinsOspA proteinBorrelia burgdorferiBorrelia cultureLyme diseaseqPCRreal-time PCRtickswildlife

Identifiers

PMID39770294
PMCPMC11679815

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.