Evidence map›Paper›PMID 39774716›Full record

ArticleCalcified tissue international2025

Adipose-Derived Stromal Cell Conditioned Medium on Bone Remodeling: Insights from a 3D Osteoblast-Osteoclast Co-Culture Model.

Elena Della Morte, Maria Pina Notarangelo, Stefania Niada, Chiara Giannasi, Federica Fortuna, Francesca Cadelano, Elisabetta Lambertini, Roberta Piva, Anna Teresa Brini, Letizia Penolazzi

Abstract read
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In one paragraph

Article in Calcified tissue international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Elena Della Morte *Laboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157, Milan, Italy.
Maria Pina Notarangelo *Department of Neuroscience and Rehabilitation, University of Ferrara, 44121, Ferrara, Italy.
Stefania NiadaLaboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157, Milan, Italy.
Chiara GiannasiLaboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157, Milan, Italy.
Federica FortunaDepartment of Medical Sciences, University of Ferrara, 44121, Ferrara, Italy.
Francesca CadelanoLaboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157, Milan, Italy.
Elisabetta LambertiniLaboratorio centralizzato di ricerca preclinica, University of Ferrara, 44121, Ferrara, Italy.
Roberta PivaDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121, Ferrara, Italy.
Anna Teresa BriniLaboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157, Milan, Italy.
Letizia PenolazziDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121, Ferrara, Italy. pnlmlt@unife.it.ORCID 0000-0002-1293-5087

Funding

Ministero della Salute Ricerca CorrenteUniversità degli Studi di Ferrara Fondo Ateneo Ricerca Scientifica_2023University of Milan PhD program in Experimental Medicine
6 · The paper itself

Abstract

This study describes the potential of the conditioned medium (CM) from adipose-derived mesenchymal stromal cells (ASCs) to affect the response of bone cells and support bone remodeling. This was in particular assessed by an in vitro model represented by a 3D human osteoblast-osteoclast co-culture. It has been reported that the effects of ASCs are predominantly attributable to the paracrine effects of their secreted factors, that are present as soluble factors or loaded into extracellular vesicles. They may affect various biological processes, including bone turnover. Our interest was to provide further evidence to support ASC-CM as a promising cell-free therapeutic agent for the treatment of bone loss. ASC-CM was characterized using nanoparticle tracking analysis (NTA), cytofluorimetry, and proteomic analysis. Human osteoblasts (hOBs) from vertebral lamina were cultured with monocytes, as osteoclasts (hOCs) precursors, in a Rotary cell culture system for 14 days. Histochemical analysis was performed to evaluate the effect of ASC-CM on bone-specific markers such as tartrate-resistant acid phosphatase (TRAP), osteopontin (OPN), RUNX2, Collagen 1 (COL1), and mineral matrix. ASC-CM characterization confirmed the content of CD63/CD81/CD9 positive extracellular vesicles. Proteomic dataset considering bone-remodeling-related keywords identified 16 processes significantly enriched. The exposure of hOBs/hOCs aggregates to ASC-CM induced increase of OPN, COL I, and RUNX2, and significantly induced mineral matrix deposition, while significantly reducing TRAP expression. These data demonstrated that CM from ASCs contains a complex of secreted factors able to control either bone resorption or bone formation and requires further investigations to deeply analyze their potential therapeutic effects.

Indexed as

Bone RemodelingCoculture TechniquesMesenchymal Stem CellsOsteoblastsOsteoclastsAdipose TissueCells, CulturedCulture Media, ConditionedHumansProteomicsCulture Media, ConditionedAdipose-derived mesenchymal stromal cellsBone remodelingConditioned mediumOsteoblastsOsteoclasts

Identifiers

PMID39774716

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.