Evidence mapPaperPMID 39775340Full record

ArticleBiogerontology2025

The protein cargo of extracellular vesicles correlates with the epigenetic aging clock of exercise sensitive DNAmFitAge.

Bernadett György, Réka Szatmári, Tamás Ditrói, Ferenc Torma, Krisztina Pálóczi, Mirjam Balbisi, Tamás Visnovitz, Erika Koltai, Péter Nagy, Edit I Buzás and 2 more

Abstract read
In one paragraph

Article in Biogerontology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Extracellular vesicles and epigenetic aging clocks in tissue aging: an exosome-focused conceptual framework with a focus on skin.The journals of gerontology. Series A, Biological sciences and medical sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Bernadett GyörgyResearch Centre for Molecular Exercise Science, Hungarian University of Sport Science, Alkotás U. 42-48, Budapest, 1123, Hungary.ORCID 0000-0002-3787-7338
Réka SzatmáriDepartment of Molecular Immunology and Toxicology and the National Tumor Biology Laboratory, National Institute of Oncology, Ráth György U. 7-9, Budapest, 1122, Hungary.ORCID 0000-0002-1313-0766
Tamás DitróiDepartment of Molecular Immunology and Toxicology and the National Tumor Biology Laboratory, National Institute of Oncology, Ráth György U. 7-9, Budapest, 1122, Hungary.ORCID 0009-0007-8148-2900
Ferenc TormaResearch Centre for Molecular Exercise Science, Hungarian University of Sport Science, Alkotás U. 42-48, Budapest, 1123, Hungary.ORCID 0000-0003-1331-8643
Krisztina PálócziDepartment of Genetics, Cell and Immunobiology, Semmelweis University, Üllői Út 26, Budapest, 1085, Hungary.ORCID 0000-0001-7065-3582
Mirjam BalbisiInstitute of Organic Chemistry, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, Budapest, 1117, Hungary.ORCID 0000-0002-6917-6974
Tamás VisnovitzDepartment of Genetics, Cell and Immunobiology, Semmelweis University, Üllői Út 26, Budapest, 1085, Hungary.ORCID 0000-0002-7962-5083
Erika KoltaiResearch Centre for Molecular Exercise Science, Hungarian University of Sport Science, Alkotás U. 42-48, Budapest, 1123, Hungary.ORCID 0000-0002-1370-2955
Péter NagyDepartment of Molecular Immunology and Toxicology and the National Tumor Biology Laboratory, National Institute of Oncology, Ráth György U. 7-9, Budapest, 1122, Hungary.ORCID 0000-0003-3393-235X
Edit I BuzásDepartment of Genetics, Cell and Immunobiology, Semmelweis University, Üllői Út 26, Budapest, 1085, Hungary.ORCID 0000-0002-3744-206X
Steve HorvathDepartment of Biostatistics, Fielding School of Public Health, University of California Los Angeles, Los Angeles, CA, 90095, USA.ORCID 0000-0002-4110-3589
Zsolt RadákResearch Centre for Molecular Exercise Science, Hungarian University of Sport Science, Alkotás U. 42-48, Budapest, 1123, Hungary. radak.zsolt@tf.hu.ORCID 0000-0003-1297-6804

Funding

EU's Horizon 2020 Research and Innovation Programme grant No. 739593.HUN-REN Hungarian Research Network grant 1500207National Cardiovascular Laboratory Program RRF-2.3.121-2022-00003National Research, Development and Innovation Office 2022-2.1.1-NL-2022-00010National Research, Development and Innovation Office NVKP_16-1-2016-0004National Research, Development and Innovation Office TKP2021-EGA-37
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are implicated in inter-organ communication, which becomes particularly relevant during aging and exercise. DNA methylation-based aging clocks reflect lifestyle and environmental factors, while regular exercise is known to induce adaptive responses, including epigenetic adaptations. Twenty individuals with High-fitness (aged 57.7 ± 9.8 years) and twenty Medium-Low-fitness (aged 57.5 ± 9.7 years) subjects provided blood samples. EVs were isolated from the samples using a size exclusion chromatography (SEC)-based method, and their protein content was analyzed by mass spectrometry (MS). Acceleration of the biological age estimator DNAmFitAge (AgeAccelFit) was associated with the protein cargo of EVs, whereas PhenoAge and GrimAge acceleration did not show a significant relationship. This finding suggests that the epigenetic aging-modulating role of exercise may involve inter-organ communication via EVs. Set Enrichment Analysis was performed to identify enriched Gene Ontology (GO) terms for sets of proteins that were either correlated with AgeAccelFit or detected exclusively in individuals with high levels of aerobic fitness. The protein cargo of EVs further suggests that inter-organ communication influences inflammation, the immune system, cellular repair, adhesion, metabolism and coagulation. Our findings help to understand the preventive role of exercise, which could be mediated in part by EVs.

Indexed as

AgingDNA MethylationEpigenesis, GeneticExerciseExtracellular VesiclesAgedFemaleHumansMaleMiddle AgedAging clockDNAmFitAgeEpigeneticsExtracellular vesiclesPhysical exerciseProteomics

Identifiers

PMID39775340
PMCPMC11711255

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.