Evidence map›Paper›PMID 39776601›Full record

ArticleFrontiers in veterinary science2024

Deer antler reserve mesenchyme cells modified with miR-145 promote chondrogenesis in cartilage regeneration.

Boyin Jia, Xintong Han, Xin Li, Linlin Zhang, Fuquan Ma, Yusu Wang, Xue Wang, Yaru Yan, Yaxin Li, Junnan Shen and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Boyin JiaCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Xintong HanCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Xin LiCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Linlin ZhangCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Fuquan MaCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Yusu WangLaboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun, China.
Xue WangCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Yaru YanCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Yaxin LiCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Junnan ShenCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Xinran ChenCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Xinyi LiCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Qianzhen ZhangCollege of Animal Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Pengfei HuInstitute of Antler Science and Product Technology, Changchun Sci-Tech University, Changchun, China.
Rui DuLaboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Deer antler-derived reserve mesenchyme cells (RMCs) are a promising source of cells for cartilage regeneration therapy due to their chondrogenic differentiation potential. However, the regulatory mechanism has not yet been elucidated. In this study, we analyzed the role of microRNAs (miRNAs) in regulating the differentiation of RMCs and in the post-transcriptional regulation of chondrogenesis and hypertrophic differentiation at the molecular and histological levels. The results showed that RMCs showed typical MSC differentiation potentials. During chondrogenic differentiation, we obtained the expression profile of miRNAs, among which miR- 145 was the most prominent candidate as a key microRNA involved in the balance of chondral and endochondral differentiation. Knockdown of miR-145 promoted chondrogenesis and inhibited hypertrophy differentiation in RMCs. Mechanically, by prediction through online databases combined with dual-luciferase reporter assay, SOX9 was suggested as a target of miR-145. Further validation experiments confirmed that knockdown of miR-145 contributed to the balance between endochondral versus chondral differentiation of RMCs by targeting SOX9. Additionally, RMCs transfected with the miR-145-knockdown-mediated lentiviral vector successfully promoted cartilage regeneration

Indexed as

cartilage regenerationchondrogenesismiR-145reserve mesenchyme cellsSOX9

Identifiers

PMID39776601
PMCPMC11705092

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.