Evidence map›Paper›PMID 39779320›Full record

ArticleEnvironmental microbiology2025

Mosquitoes Reared in Nearby Insectaries at the Same Institution Have Significantly Divergent Microbiomes.

Laura E Brettell, Ananya F Hoque, Tara S Joseph, Vishaal Dhokiya, Emily A Hornett, Grant L Hughes, Eva Heinz

Abstract read
In one paragraph

Article in Environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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  7. Microbial gatekeepers: midgut bacteria inFrontiers in microbiology · 2025
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Laura E BrettellDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID https://orcid.org/0000-0002-6050-4804
Ananya F HoqueDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID https://orcid.org/0000-0002-0964-4414
Tara S JosephDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
Vishaal DhokiyaDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID https://orcid.org/0000-0002-7733-1356
Emily A HornettDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID https://orcid.org/0000-0002-7747-0583
Grant L HughesDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID https://orcid.org/0000-0002-7567-7185
Eva HeinzDepartment of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID https://orcid.org/0000-0003-4413-3756

Funding

Exploiting gnotobiotic systems to examine microbiota acquisition in mosquitoes and for microbiome transplantationsR21AI138074 · NIAID · TMLIVERPOOL SCHOOL OF TROPICAL MEDICINE · PI HUGHES, GRANT LESLIE · 2018 to 2019
$296k
Bill and Melinda Gates Foundation INV-048598Biotechnology and Biological Sciences Research Council BB/T001240/1Biotechnology and Biological Sciences Research Council BB/V011278/1Biotechnology and Biological Sciences Research Council BB/V011278/2Biotechnology and Biological Sciences Research Council BB/W018446/1Biotechnology and Biological Sciences Research Council BB/X018024/1Engineering and Physical Sciences Research Council V043811/1Liverpool School of Tropical Medicine Director's Catalyst FundMedical Research Council MR/N013514/1National Institute for Health and Care Research NIHR2000907NIAID NIH HHS R21 AI138074NIH HHS R21AI138074Royal Society RSWF\R1\180013UK Research and Innovation 20197UK Research and Innovation 85336Wellcome TrustWellcome Trust 217303/Z/19/Z
6 · The paper itself

Abstract

The microbiome influences critical aspects of mosquito biology and variations in microbial composition can impact the outcomes of laboratory studies. To investigate how biotic and abiotic conditions in an insectary affect the composition of the mosquito microbiome, a single cohort of Aedes aegypti eggs was divided into three batches and transferred to three different climate-controlled insectaries within the Liverpool School of Tropical Medicine. The bacterial microbiome composition was compared as mosquitoes developed, the microbiome of the mosquitoes' food sources was characterised, environmental conditions over time in each insectary were measured, and mosquito development and survival were recorded. While developmental success was similar across all three insectaries, differences in microbiome composition were observed between mosquitoes from each insectary. Environmental conditions and bacterial input via food sources varied between insectaries, potentially contributing to the observed differences in microbiome composition. At both adult and larval stages, specific members of the mosquito microbiome were associated with particular insectaries; the insectary with less stable and cooler conditions resulted in a slower pupation rate and higher diversity of the larval microbiome. These findings underscore that even minor inconsistencies in rearing conditions can affect the composition of the mosquito microbiome, which may influence experimental outcomes.

Indexed as

AedesLarvaMicrobiotaAnimalsBacteriaFemaleRNA, Ribosomal, 16SRNA, Ribosomal, 16SAedesdevelopmentdiversityenvironmenthumiditymicrobiometemperature

Identifiers

PMID39779320
PMCPMC11711076

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.