Evidence map›Paper›PMID 39794843›Full record

ArticleCell communication and signaling : CCS2025

In vitro effect of hCG on cryptorchid patients' gubernacular cells: a predictive model for adjuvant personalized therapy.

Andrea Errico, Giulia Ambrosini, Sara Vinco, Emanuela Bottani, Elisa Dalla Pozza, Nunzio Marroncelli, Jessica Brandi, Daniela Cecconi, Ilaria Decimo, Filippo Migliorini and 2 more

Abstract read
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In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Andrea ErricoDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
Giulia AmbrosiniDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
Sara VincoDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
Emanuela BottaniDepartment of Diagnostics and Public Health, University of Verona, Verona, Italy.
Elisa Dalla PozzaDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
Nunzio MarroncelliDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
Jessica BrandiDepartment of Biotechnology, University of Verona, Verona, Italy.
Daniela CecconiDepartment of Biotechnology, University of Verona, Verona, Italy.
Ilaria DecimoDepartment of Diagnostics and Public Health, University of Verona, Verona, Italy.
Filippo MiglioriniUOC of Urology, Azienda Ospedaliera Universitaria Integrata di Verona, Verona, Italy.
Nicola ZampieriDepartment of Engineering and Innovation Medicine, Paediatric Fertility Lab, Woman and Child Hospital, Division of Pediatric Surgery, University of Verona, Verona, Italy. nicola.zampieri@aovr.veneto.it.
Ilaria DandoDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy. ilaria.dando@univr.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCryptorchidism is the absence of one or both testicles in the scrotum at birth, being a risk factor for testis cancer and infertility. The most effective method to treat cryptorchidism is orchiopexy, followed by human chorionic gonadotropin (hCG) therapy; however, a portion of treated patients do not show a significant improvement in testis volume and vascularization after adjuvant therapy.

methodsIn this study, we generated an in vitro model to predict the patient response to hCG by cultivating and treating primary cells derived from five cryptorchid patients' biopsies of gubernaculum testis, the ligament that connects the testicle to the scrotum. On these in vitro cultured cells, we analyzed the effect of hCG on cell proliferation, tubular structure formation, cellular respiration, reactive oxygen species content, and proteome.

resultsWe demonstrate that in vitro hCG stimulates gubernacular cells to proliferate and form vessel-like structures to a different extent among the five cryptorchid patients' cells, with a decrease in oxygen consumption and reactive oxygen species generation. Furthermore, from the proteomic analysis, we show that hCG regulates the intra- and extra-cellular organization of gubernacular cells together with a massive regulation of the antioxidant response.

conclusionsHereby, we characterized the cellular and molecular effects of hCG, demonstrating that the diverse patient response to hCG may be ascribable to their age since young patients better respond in vitro to the hormone, supporting a prompt surgical procedure and subsequent therapy.

trial registrationThe study was conducted in accordance with the Declaration of Helsinki and approved by the Institutional Ethics Committee of "Azienda Ospedaliera Universitaria Integrata" (AOUI) of Verona, Italy ("ANDRO-PRO", protocol code N. 4206 CESC of 26 April 2023).

Indexed as

Chorionic GonadotropinCryptorchidismGubernaculumPrecision MedicineAdolescentAdultAndrogensBiopsyCell ProliferationCells, CulturedChildChild, PreschoolGonadotropinsHumansInfantMaleAndrogensChorionic GonadotropinGonadotropinsReactive Oxygen SpeciesCryptorchidismEnergetic metabolismGubernaculum testisHuman chorionic gonadotropinOxidative stress

Identifiers

PMID39794843
PMCPMC11724491

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.