Evidence map›Paper›PMID 39796006›Full record

ArticleInternational journal of molecular sciences2024

Characterization of Mesenchymal and Neural Stem Cells Response to Bipolar Microsecond Electric Pulses Stimulation.

Giorgia Innamorati, Marina Sanchez-Petidier, Giulia Bergafora, Camilla Codazzi, Valentina Palma, Francesca Camera, Caterina Merla, Franck M André, Maria Pedraza, Victoria Moreno Manzano and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Neural Circuit Mapping and Neurotherapy-Based Strategies.Cellular and molecular neurobiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Giorgia InnamoratiPhD Program in Cellular and Molecular Biology, Department of Biology, University of Rome "Tor Vergata", 00133 Rome, Italy.
Marina Sanchez-PetidierNeural Circuits and Behaviour Laboratory, Fundación Hospital Nacional de Parapléjicos, 45004 Toledo, Spain.
Giulia BergaforaDivision of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.
Camilla CodazziDivision of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.
Valentina PalmaDivision of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.
Francesca CameraDivision of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0001-6455-4094
Caterina MerlaDivision of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0001-8612-9566
Franck M AndréMetabolic and Systemic Aspects of the Oncogenesis (METSY), CNRS, Institut Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France.ORCID 0000-0001-6297-1433
Maria PedrazaNeuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe (CIPF), 46012 Valencia, Spain.ORCID 0000-0002-1973-3269
Victoria Moreno ManzanoNeuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe (CIPF), 46012 Valencia, Spain.ORCID 0000-0002-6035-9491
Laura CaramazzaBioEMLab Group, DIET, Department of Information Engineering, Electronics and Telecommunications Sapienza, University of Rome, 00184 Rome, Italy.ORCID 0000-0002-9304-3477
Micol ColellaBioEMLab Group, DIET, Department of Information Engineering, Electronics and Telecommunications Sapienza, University of Rome, 00184 Rome, Italy.ORCID 0000-0003-1715-9619
Paolo MarracinoRise Technology Srl, 00121 Rome, Italy.ORCID 0000-0003-4742-8170
Marco BalucaniRise Technology Srl, 00121 Rome, Italy.
Francesca ApollonioBioEMLab Group, DIET, Department of Information Engineering, Electronics and Telecommunications Sapienza, University of Rome, 00184 Rome, Italy.ORCID 0000-0001-6937-9731
Micaela LibertiBioEMLab Group, DIET, Department of Information Engineering, Electronics and Telecommunications Sapienza, University of Rome, 00184 Rome, Italy.ORCID 0000-0002-7494-2696
Claudia ConsalesDivision of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0002-7805-4493

Funding

H2020 FET-OPEN 964562
6 · The paper itself

Abstract

In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.

Indexed as

Electric StimulationMesenchymal Stem CellsNeural Stem CellsAnimalsApoptosisCell CycleCell DifferentiationCell ProliferationCells, CulturedHumanscell proliferationgene expressionmicrosecond electric pulse stimulationstem cells

Identifiers

PMID39796006
PMCPMC11720446

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.