Evidence map›Paper›PMID 39799319›Full record

ArticleMolecular cancer2025

Mir-483-5p-mediated activating of IGF2/H19 enhancer up-regulates IGF2/H19 expression via chromatin loops to promote the malignant progression of hepatocellular carcinoma.

Weiwei Chen, Chutian Wu, Yuting Li, Tonghua Wang, Miaoling Huang, Min Wang, Linjing Long, Yanfang Chen, Shufen Feng, Xuyou Liu and 1 more

Abstract read
In one paragraph

Article in Molecular cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Weiwei Chen *Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China.
Chutian Wu *Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China.
Yuting Li *Department of Gastroenterology, Weifang People's Hospital, Shandong Second Medical University, Shandong, China.
Tonghua Wang *Department of Gastroenterology, Affiliated Hospital of Youjiang Medical University for Nationalities, BaiSe, P. R. China.
Miaoling Huang *Department of General Practice, The First Affiliated Hospital, Jinan University, Guangzhou, P. R. China.
Min Wang *Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China.
Linjing LongDepartment of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China.
Yanfang ChenDepartment of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China.
Shufen FengDepartment of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China.
Xuyou LiuDepartment of Gastroenterology, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, P. R. China. liuxy717@163.com.
Shaohui TangDepartment of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510630, P. R. China. tangshaohui206@163.com.

Funding

Funding by Science and Technology Projects in Guangzhou No. 2023A04J0634Funds of the Department of Science and Technology of Guizhou Province No. ZK [2023] 484the National Natural Science Foundation of China No. 81972211Youjiang Medical College for Nationalities Affiliated Hospital high-level talent research project talent special No.R202011703
6 · The paper itself

Abstract

backgroundThe insulin-like growth factor 2 (IGF2) and H19 are overexpressed in hepatocellular carcinoma (HCC). IGF2-derived miR-483-5p is implicated in the development of cancers. Here, we investigated the involvement of miR-483-5p in IGF2 and H19 overexpression regulation and its role in HCC.

methodsFirstly, the effect of miR-483-5p on the expression of IGF2 and H19, and the binding of miR-483-5p to IGF2/H19 enhancer were evaluated in HCC cells. Next, miR-483-5p-mediated IGF2/H19 enhancer activation and its mechanism were investigated in HCC cells. Then, the mechanism by which active IGF2/H19 enhancer mediated by miR-483-5p activate IGF2/H19 promoters was studied in HCC cells. Finally, the effect of MED1 on the expression of IGF2/H19 as well as the malignant phenotype of HCC cells in vitro and in vivo mediated by miR-483-5p was evaluated.

resultsMir-483-5p up-regulated IGF2 P2 mRNA-P4 mRNA and H19 expression by binding to IGF2/H19 enhancer resulting in IGF2/H19 enhancer activation in HCC cells. Mechanistically, miR-483-5p increased recruitment of Ago1 and Ago2 at IGF2/H19 enhancer and then activated transcription of IGF2/H19 eRNA by RNA polymerase II and p300, which further induced chromatin loops formation between IGF2/H19 enhancer and IGF2/H19 promoters to activate IGF2/H19 promoters via IGF2/H19 eRNA-MED1-IGF2/H19 promoters complex in HCC cells. In this process, MED1 promoted chromatin loops formation as well as the malignant phenotype of HCC cells in vitro and in vivo mediated by miR-483-5p.

conclusionsmiR-483-5p-mediated activating of IGF2/H19 enhancer up-regulates IGF2/H19 expression via DNA loops, thereby promoting the malignant progression of HCC.

Indexed as

Carcinoma, HepatocellularChromatinEnhancer Elements, GeneticGene Expression Regulation, NeoplasticInsulin-Like Growth Factor IILiver NeoplasmsMicroRNAsRNA, Long NoncodingAnimalsCell Line, TumorDisease ProgressionHumansMicePromoter Regions, GeneticUp-RegulationChromatinH19 long non-coding RNAIGF2 protein, humanInsulin-Like Growth Factor IIMicroRNAsMIRN483 microRNA, humanRNA, Long NoncodingEnhancerHCCMED1miR-483-5p

Identifiers

PMID39799319
PMCPMC11724483

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.