Evidence map›Paper›PMID 39806157›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Mechanistic Insights into Synaptotagmin-1 Mediated Membrane Fusion and Interactions.

Merve Sari, Alina Schmidt, Jörn Dietz, Claudia Steinem, Andreas Janshoff

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Merve SariInstitutes of Physical Chemistry, and Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany.
Alina SchmidtInstitute of Physical Chemistry, University of Göttingen, Göttingen, Germany.
Jörn DietzInstitute of Physical Chemistry, University of Göttingen, Göttingen, Germany.
Claudia SteinemInstitutes of Physical Chemistry, and Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany.
Andreas JanshoffInstitute of Physical Chemistry, University of Göttingen, Göttingen, Germany. Andreas.Janshoff@chemie.uni-goettingen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present two innovative approaches to investigate the dynamics of membrane fusion and the strength of protein-membrane interactions. The first approach employs pore-spanning membranes (PSMs), which allow for the observation of protein-assisted fusion processes. The second approach utilizes colloidal probe microscopy with membrane-coated probes with reconstituted proteins. PSMs enable one to obtain detailed information about the fusion process with particular emphasis on fusion intermediates and fusion pore formation. We demonstrate the potential of the PSM system using SNARE-mediated fusion. Accompanied by colloidal probe microscopy, molecular information can be gathered on how full-length synaptotagmin-1 (syt-1) contributes to the fusion process. We propose that syt-1 engages with anionic bilayers, significantly modifying the adhesion between membranes. The introduction of Ca

Indexed as

Membrane FusionSynaptotagmin IAnimalsCalciumHumansLipid BilayersProtein BindingSNARE ProteinsCalciumLipid BilayersSNARE ProteinsSynaptotagmin IColloidal probe microscopyFluorescence microscopyPore-spanning membranesSNAREsSupported lipid bilayersSynaptotagmin

Identifiers

PMID39806157

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.