Evidence mapPaperPMID 39806246Full record

ArticleMolecular biology reports2025

Upregulated PCAT-1 predicts poor prognosis and reduced immune cell infiltration in head and neck squamous cell carcinoma through the miR-145-5p / FSCN-1 axis.

Maryam Pirhoushiaran, Roozbeh Heidarzadehpilehrood, Mahnoosh Mokhtarinejad, Sara Hesami, Nima Rezaei, Abbas Shakoori Farahani

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Maryam PirhoushiaranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran.ORCID http://orcid.org/0000-0002-8863-3237
Roozbeh HeidarzadehpilehroodDepartment of Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.ORCID http://orcid.org/0000-0001-7420-0599
Mahnoosh MokhtarinejadDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran.
Sara HesamiMedical Genetic Ward, Faculty of Medicine, Imam Khomeini Hospital Complex, IKHC, Tehran University of Medical Sciences, Tehran, Iran.ORCID http://orcid.org/0000-0002-2048-0633
Nima RezaeiResearch Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.ORCID http://orcid.org/0000-0002-3836-1827
Abbas Shakoori FarahaniMedical Genetic Ward, Faculty of Medicine, Imam Khomeini Hospital Complex, IKHC, Tehran University of Medical Sciences, Tehran, Iran. shakooria@tums.ac.ir.ORCID http://orcid.org/0000-0002-4363-1967

Funding

Tehran University of Medical Sciences (TUMS) 98-01-30-39989
6 · The paper itself

Abstract

backgroundLncRNA PCAT-1 is known to promote cancer proliferation, invasion, and metastasis. However, its significance in HNSCC is not fully understood. This research investigates how the PCAT-1 / miR-145-5p / FSCN-1 axis promote HNSCC. METHODS AND

resultsWe analyzed the gene expression patterns on 238 fresh-frozen samples, comparing tumors with their normal adjacent tissues (NATs). HNSCC samples showed higher PCAT-1 and FSCN-1 expression compared to NATs (p < 0.001 and p < 0.001, respectively). In contrast, miR-145-5p expression was markedly downregulated compared to NATs (p < 0.001). Notably, ROC curve analysis revealed exceptional diagnostic power, with an AUC of 0.83 for PCAT-1, 0.95 for miR-145-5p, and 0.91 for FSCN-1. Pearson correlation analysis unveiled a significant positive correlation between PCAT-1 and FSCN-1 expression levels (r = 0.084, p < 0.001) and negative correlations between FSCN-1 and miR-145-5p (r = -0.710, p < 0.001) as well as between PCAT-1 and miR-145-5p (r = -0.759, p < 0.001). Distinct molecular profiles were observed in the levels of PCAT-1, miR-145-5p, and FSCN-1 between HPV (-) and HPV ( +) 16 and 18 genotypes (p = 0.007, p = 0.027, and p = 0.002). MiR-145-5p expression showed significant differences between HPV (-) and HPV ( +) other genotypes (p = 0.035). FSCN-1 expression showed notable distinctions between HPV ( +) 18 & 16 and HPV ( +) other genotypes (p = 0.031).

conclusionsElevated levels of lncRNA PCAT-1 promote HNSCC through the miR-145-5p/FSCN-1 axis and are associated with poor prognosis and reduced immune cell infiltration levels.

Indexed as

Carrier ProteinsHead and Neck NeoplasmsMicrofilament ProteinsMicroRNAsRNA, Long NoncodingSquamous Cell Carcinoma of Head and NeckAgedBiomarkers, TumorFemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedPrognosisUp-RegulationBiomarkers, TumorCarrier ProteinsFSCN1 protein, humanlong non-coding RNA PCAT1, humanMicrofilament ProteinsMicroRNAsMIRN145 microRNA, humanRNA, Long NoncodingFSCN-1Head and neck squamous cell carcinomaHPV genotypemiRNA-145-5pPCAT-1SmokingTumor infiltration

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.