Evidence map›Paper›PMID 39806329›Full record

ArticleBMC plant biology2025

Fine mapping and expression characteristics analysis of male-sterile gene BrRNR1 in Chinese cabbage (Brassica rapa L. ssp. pekinensis).

Meihui Xue, Jiahang Li, Ruiqi Liao, Junjie Xu, Mingwei Zhou, Runpeng Yao, Zhiyong Liu, Hui Feng, Shengnan Huang

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In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Advances in mutant characterization for detecting causal mutations in crop plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Meihui Xue *College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Jiahang Li *College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Ruiqi LiaoCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Junjie XuCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Mingwei ZhouCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Runpeng YaoTonghua Horticulture Research Institute, Tonghua, 134000, China.
Zhiyong LiuCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Hui FengCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Shengnan HuangCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China. hsn870806@syau.edu.cn.

Funding

Key Research and Development Project of Liaoning Province, China 2024JH2/102500048National Natural Science Foundation of China 31801854
6 · The paper itself

Abstract

backgroundChinese cabbage is a cross-pollinated crop with remarkable heterosis, and male-sterile line is an important mean to produce its hybrids. In this study, a male-sterile mutant msm7 was isolated from a Chinese cabbage DH line 'FT' by using EMS-mutagenesis.

resultsCompared with the wild-type 'FT', the anthers of mutant msm7 were completely aborted, accompanied by the defects in leaf and petal development. Genetic analysis showed that a single recessive nuclear gene controlled the sterile phenotype of mutant msm7. Cytological observation indicated that the anther abortion of mutant msm7 was caused by the degenarated microspores and premature degradation of tapetum. MutMap and KASP analyses identified that BraA01g038270.3 C, encoding the large subunit of ribonucleotide reductase (RNR1) which involved in the biosynthesis of dNTPs, was the candidate gene, named BrRNR1. Compared with the wild-type 'FT', a G-A mutation occurred on the 4th exon of the BrRNR1 gene, leading to the premature termination of encoded amino acid in mutant msm7. Expression analysis indicated that the BrRNR1 gene was ubiquitously expressed in all organs and was significantly decreased in flower bud and anther of mutant msm7 compared with the wild-type 'FT'. Subcellular localization revealed that BrRNR1 was an endoplasmic reticulum localization protein.

conclusionOur study is the first to characterize the function of BrRNR1 gene associated with male sterility and lays a foundation for exploring the molecular mechanism of anther abortion caused by the mutation in BrRNR1 gene of Chinese cabbage.

Indexed as

Brassica rapaGenes, PlantPlant InfertilityPlant ProteinsChromosome MappingFlowersGene Expression Regulation, PlantMutationPlant ProteinsChinese cabbageFine mappingMale sterilityMutationRibonucleotide reductase

Identifiers

PMID39806329
PMCPMC11730134

What Socratic holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.