Evidence map›Paper›PMID 39814746›Full record

ArticleNature communications2025

Single-cell analysis identifies the CNP/GC-B/cGMP axis as marker and regulator of modulated VSMCs in atherosclerosis.

Moritz Lehners, Hannes Schmidt, Maria T K Zaldivia, Daniel Stehle, Michael Krämer, Andreas Peter, Julia Adler, Robert Lukowski, Susanne Feil, Robert Feil

Abstract read
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Perspectives on C-Type Natriuretic Peptide in Cardiometabolic Disease.Journal of the American Heart Association · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Moritz LehnersInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany.ORCID http://orcid.org/0000-0002-3841-3930
Hannes SchmidtInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany.
Maria T K ZaldiviaInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany.
Daniel StehleInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany.ORCID http://orcid.org/0000-0001-9086-2306
Michael KrämerInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany.
Andreas PeterDepartment for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital Tübingen, Tübingen, Germany.ORCID http://orcid.org/0000-0001-5732-2287
Julia AdlerDepartment of Pharmacology, Toxicology and Clinical Pharmacy, Institute of Pharmacy, University of Tübingen, Tübingen, Germany.
Robert LukowskiDepartment of Pharmacology, Toxicology and Clinical Pharmacy, Institute of Pharmacy, University of Tübingen, Tübingen, Germany.ORCID http://orcid.org/0000-0002-4564-3574
Susanne FeilInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany.ORCID http://orcid.org/0000-0003-2148-8773
Robert FeilInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Germany. robert.feil@uni-tuebingen.de.ORCID http://orcid.org/0000-0002-7335-4841

Funding

EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) ERA-CVD JTC2017-044
6 · The paper itself

Abstract

A balanced activity of cGMP signaling contributes to the maintenance of cardiovascular homeostasis. Vascular smooth muscle cells (VSMCs) can generate cGMP via three ligand-activated guanylyl cyclases, the NO-sensitive guanylyl cyclase, the atrial natriuretic peptide (ANP)-activated GC-A, and the C-type natriuretic peptide (CNP)-stimulated GC-B. Here, we study natriuretic peptide signaling in murine VSMCs and atherosclerotic lesions. Correlative profiling of pathway activity and VSMC phenotype at the single-cell level shows that phenotypic modulation of contractile VSMCs to chondrocyte-like plaque cells during atherogenesis is associated with a switch from ANP/GC‑A to CNP/GC‑B signaling. Silencing of the CNP/GC-B axis in VSMCs results in an increase of chondrocyte-like plaque cells. These findings indicate that the CNP/GC-B/cGMP pathway is a marker and atheroprotective regulator of modulated VSMCs, limiting their transition to chondrocyte-like cells. Overall, this study highlights the plasticity of cGMP signaling in VSMCs and suggests analogies between CNP-dependent remodeling of bone and blood vessels.

Indexed as

AtherosclerosisCyclic GMPMuscle, Smooth, VascularMyocytes, Smooth MuscleNatriuretic Peptide, C-TypeReceptors, Atrial Natriuretic FactorAnimalsAtrial Natriuretic Factor Receptor BBiomarkersMaleMiceMice, Inbred C57BLSignal TransductionSingle-Cell AnalysisAtrial Natriuretic Factor Receptor BBiomarkersCyclic GMPNatriuretic Peptide, C-TypeReceptors, Atrial Natriuretic Factor

Identifiers

PMID39814746
PMCPMC11735800

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.