Evidence map›Paper›PMID 39815745›Full record

ArticleJournal of pathology and translational medicine2025

PLUNC downregulates the expression of PD-L1 by inhibiting the interaction of DDX17/β-catenin in nasopharyngeal carcinoma.

Ranran Feng, Yilin Guo, Meilin Chen, Ziying Tian, Yijun Liu, Su Jiang, Jieyu Zhou, Qingluan Liu, Xiayu Li, Wei Xiong and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pathology and translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Role of DEAD/DEAH-box helicases in immunity, infection and cancers.Cell communication and signaling : CCS · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Ranran FengDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Yilin GuoDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Meilin ChenThe First Affiliated Hospital of Sun Yatsen University, Guangzhou, China.
Ziying TianDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Yijun LiuDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Su JiangDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Jieyu ZhouDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Qingluan LiuDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Xiayu LiHunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, China.
Wei XiongNHC Key Laboratory of Carcinogenesis, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
Lei ShiDepartment of Pathology, Second Xiangya Hospital, Central South University, Changsha, China.
Songqing FanDepartment of Pathology, Second Xiangya Hospital, Central South University, Changsha, China.
Guiyuan LiNHC Key Laboratory of Carcinogenesis, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
Wenling ZhangDepartment of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.

Funding

Hunan Province Natural Science Foundation 2020JJ4780National Natural Science Foundation of China 81672688
6 · The paper itself

Abstract

backgroundNasopharyngeal carcinoma (NPC) is characterized by high programmed death-ligand 1 (PD-L1) expression and abundant infiltration of non-malignant lymphocytes, which renders patients potentially suitable candidates for immune checkpoint blockade therapies. Palate, lung, and nasal epithelium clone (PLUNC) inhibit the growth of NPC cells and enhance cellular apoptosis and differentiation. Currently, the relationship between PLUNC (as a tumor-suppressor) and PD-L1 in NPC is unclear.

methodsWe collected clinical samples of NPC to verify the relationship between PLUNC and PD-L1. PLUNC plasmid was transfected into NPC cells, and the variation of PD-L1 was verified by western blot and immunofluorescence. In NPC cells, we verified the relationship of PD-L1, activating transcription factor 3 (ATF3), and β-catenin by western blot and immunofluorescence. Later, we further verified that PLUNC regulates PD-L1 through β-catenin. Finally, the effect of PLUNC on β-catenin was verified by co-immunoprecipitation (Co-IP).

resultsWe found that PLUNC expression was lower in NPC tissues than in paracancer tissues. PD-L1 expression was opposite to that of PLUNC. Western blot and immunofluorescence showed that β-catenin could upregulate ATF3 and PD-L1, while PLUNC could downregulate ATF3/PD-L1 by inhibiting the expression of β-catenin. PLUNC inhibits the entry of β-catenin into the nucleus. Co-IP experiments demonstrated that PLUNC inhibited the interaction of DEAD-box helicase 17 (DDX17) and β-catenin.

conclusionsPLUNC downregulates the expression of PD-L1 by inhibiting the interaction of DDX17/β-catenin in NPC.

Indexed as

BPIFA1 proteinDEAD-box RNA helicases 17Programmed cell death 1 ligand 1

Identifiers

PMID39815745
PMCPMC11736280

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.