Evidence map›Paper›PMID 39821901›Full record

ArticleJournal of fish diseases2025

Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses.

Torben Krebs, Julia Bauer, Sarah Graff, Lukas Teich, Markus Sterneberg, Marina Gebert, Henrike Seibel, Bettina Seeger, John Hellmann, Øystein Wessel and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fish diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Torben KrebsFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Julia BauerFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Sarah GraffWorking Group Fish Health and -Welfare, Section Aquaculture and Aquatic Resources, Fraunhofer Research Institution for Individualized and Cell-Based Medical Engineering IMTE, Büsum, Germany.
Lukas TeichFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Markus SternebergFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Marina GebertWorking Group Fish Health and -Welfare, Section Aquaculture and Aquatic Resources, Fraunhofer Research Institution for Individualized and Cell-Based Medical Engineering IMTE, Büsum, Germany.
Henrike SeibelWorking Group Fish Health and -Welfare, Section Aquaculture and Aquatic Resources, Fraunhofer Research Institution for Individualized and Cell-Based Medical Engineering IMTE, Büsum, Germany.
Bettina SeegerInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany.ORCID https://orcid.org/0000-0002-4653-2841
John HellmannEnvironment and Consumer Protection, Fisheries Ecology and Aquaculture, North Rhine Westphalian State Agency for Nature, Germany.
Øystein WesselFaculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.ORCID https://orcid.org/0000-0002-0811-7305
Espen RimstadFaculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.ORCID https://orcid.org/0000-0001-9911-5948
Win SurachetpongDepartment of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.ORCID https://orcid.org/0000-0002-5707-3476
Dieter SteinhagenFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Verena Jung-SchroersFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Mikolaj AdamekFish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.ORCID https://orcid.org/0000-0003-4890-3164

Funding

Bundesministerium für Bildung und Forschung
6 · The paper itself

Abstract

Piscine orthoreovirus-1 and 3 (PRV-1, PRV-3) cause highly prevalent infection in cultured salmonids and can induce heart and skeletal muscle inflammation (HSMI) resulting in economic losses in aquaculture. However, to date, PRV-1 and PRV-3 have withstood replication in continuous cell lines. In this study, we used beating heart cell cultures obtained from different developmental stages of rainbow trout (Oncorhynchus mykiss) (RTC-L and RTC-A) and tested their ability to sustain replication of PRV-1 and PRV-3. Furthermore, we compared the replication pattern of the different viruses with those in the newly developed heart fibroblast cell line (RTH-F) and the traditional established rainbow trout gonad cell line (RTG-2). Neither RTCs nor RTH-F cell lines supported replication of PRV-1 and PRV-3. Comparative experiments showed varying susceptibility of the novel cultures to viral haemorrhagic septicaemia virus (VHSV), chum salmon reovirus (CSV), infectious pancreatic necrosis virus (IPNV), piscine myocarditis virus (PMCV), salmonid alphavirus 3 (SAV-3) and tilapia lake virus (TiLV), indicating their usability for work with multiple fish viruses. While confirming the difficulty of replicating PRV-1 and PRV-3, the results demonstrate the potential of novel heart-derived cell cultures as in vitro tools for studying fish viruses.

Indexed as

Fish DiseasesHeartOncorhynchus mykissOrthoreovirusVirus ReplicationAnimalsCell LineCells, Culturedcell culturesheartPRV‐1rainbow troutvirus

Identifiers

PMID39821901
PMCPMC11976189

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.