Evidence map›Paper›PMID 39824554›Full record

ArticleMethods in cell biology2025

Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications.

Varsha Gadiyar, David C Calianese, Rachael Pulica, Christopher Varsanyi, Ziren Wang, Ahmed Aquib, Alok Choudhary, Raymond B Birge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Varsha GadiyarDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States.
David C CalianeseDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States; Laboratory of Biochemistry and Immunology, World Premier International Research Center, Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
Rachael PulicaDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States.
Christopher VarsanyiDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States.
Ziren WangDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States.
Ahmed AquibDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States.
Alok ChoudharyDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ, United States. Electronic address: choudhak@njms.rutgers.edu.
Raymond B BirgeDepartment of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States. Electronic address: birgera@njms.rutgers.edu.

Funding

Targeting a phosphatidylserine/TAM receptor/PD-L1 axis as a vulnerability in cancerR01CA260137 · NCI · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI Raymond B. Birge, SERGEI V KOTENKO · 2022 to 2026
$2.4M
NCI NIH HHS R01 CA260137
6 · The paper itself

Abstract

The externalization of Phosphatidylserine (PS) from the inner surface of the plasma membrane to the outer surface of the plasma membrane is an emblematic event during apoptosis and serves as a potent "eat-me" signal for the efferocytosis of apoptotic cells. Although less well understood, PS is also externalized on live cells in the tumor microenvironment and on live virus-infected cells whereby it serves as an immune modulatory signal that drives tolerance and immune escape. Given the importance of PS in cancer immunology and immune escape, PS-targeting monoclonal antibodies have been characterized with promising immunotherapeutic potential. Here, we describe the cloning and characterization of a series of PS targeting antibodies and their potential use and utility in immuno-oncology.

Indexed as

Antibodies, MonoclonalPhosphatidylserinesAnimalsApoptosisCell MembraneCloning, MolecularHumansImmunotherapyAntibodies, MonoclonalPhosphatidylserines11.311N11Annexin VAntibody purificationBavituximabFlow cytometryImmuno-oncology applicationsMonoclonal antibodiesPhosphatidylserine ELISAPhosphatidylserine externalizationβ2GPI

Identifiers

PMID39824554
PMCPMC12831197

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.