ArticleAdvanced biology2025
Light-Triggered Protease-Mediated Release of Actin-Bound Cargo from Synthetic Cells.
Article in Advanced biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Let There be Light! Light as an Engine and Regulator in Synthetic Cells.Angewandte Chemie (International ed. in English) · 2026Review
- Light-Controlled Membrane Fusion in Synthetic Cells.Life (Basel, Switzerland) · 2026Review
- Light-controlled synthetic communication networks via paired connexon nanopores.Nature communications · 2025Article
- pH-responsive synthetic cells for controlled protein synthesis and release.bioRxiv : the preprint server for biology · 2025Article
- Tau-Driven Coordination of Microtubule-Actin Crosstalk in Cell-Sized Vesicles.bioRxiv : the preprint server for biology · 2025Article
- Light-Triggered Protease-Mediated Release of Actin-Bound Cargo from Synthetic Cells.Advanced biology · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Synthetic cells offer a versatile platform for addressing biomedical and environmental challenges, due to their modular design and capability to mimic cellular processes such as biosensing, intercellular communication, and metabolism. Constructing synthetic cells capable of stimuli-responsive secretion is vital for applications in targeted drug delivery and biosensor development. Previous attempts at engineering secretion for synthetic cells have been confined to non-specific cargo release via membrane pores, limiting the spatiotemporal precision and specificity necessary for selective secretion. Here, a protein-based platform termed TEV Protease-mediated Releasable Actin-binding Protein (TRAP) is designed and constructed for selective, rapid, and triggerable secretion in synthetic cells. TRAP is designed to bind tightly to reconstituted actin networks and is proteolytically released from bound actin, followed by secretion via cell-penetrating peptide membrane translocation. TRAP's efficacy in facilitating light-activated secretion of both fluorescent and luminescent proteins is demonstrated. By equipping synthetic cells with a controlled secretion mechanism, TRAP paves the way for the development of stimuli-responsive biomaterials, versatile synthetic cell-based biosensing systems, and therapeutic applications through the integration of synthetic cells with living cells for targeted delivery of protein therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.