Evidence map›Paper›PMID 39829936›Full record

ArticlebioRxiv : the preprint server for biology2025

Decoding Gene Networks Controlling Hypothalamic and Prethalamic Neuron Development.

Dong Won Kim, Leighton H Duncan, Jenny Xu, Minzi Chang, Sara Sejer Sørensen, Chantelle E Terrillion, Patrick O Kanold, Elsie Place, Seth Blackshaw

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Dong Won KimDanish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Aarhus, Denmark.
Leighton H DuncanSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jenny XuDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Minzi ChangDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sara Sejer SørensenDanish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Aarhus, Denmark.
Chantelle E TerrillionDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Patrick O KanoldSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Elsie PlaceSchool of Biosciences, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.
Seth BlackshawSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID 0000-0002-1338-8476

Funding

NEURONAL CIRCUITS AND PLASTICITY OF NEONATAL AUDITORY CORTEXR01DC009607 · NIDCD · UNIV OF MARYLAND, COLLEGE PARK · PI PATRICK O KANOLD · 2009 to 2026
$7.8M
Development and function of hypothalamic Lhx6-positive neuronsR01MH126676 · NIMH · JOHNS HOPKINS UNIVERSITY · PI BLACKSHAW, SETH · 2021 to 2025
$2.0M
Intrinsic and Extrinsic factors regulating neurogenic competence in hypothalamic tanycytesF99NS135816 · NINDS · JOHNS HOPKINS UNIVERSITY · PI DUNCAN, LEIGHTON HOSEA · 2023 to 2024
$99k
Investigating molecular mechanisms and function of tanycyte-derived neurogenesis in the postnatal hypothalamusF31DK132944 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI DUNCAN, LEIGHTON HOSEA · 2022 to 2023
$70k
NIDCD NIH HHS R01 DC009607NIDDK NIH HHS F31 DK132944NIMH NIH HHS R01 MH126676NINDS NIH HHS F99 NS135816
6 · The paper itself

Abstract

Neuronal subtypes derived from the embryonic hypothalamus and prethalamus regulate many essential physiological processes, yet the gene regulatory networks controlling their development remain poorly understood. Using single-cell RNA- and ATAC-sequencing, we analyzed mouse hypothalamic and prethalamic development from embryonic day 11 to postnatal day 8, profiling 660,000 cells in total. This identified key transcriptional and chromatin dynamics driving regionalization, neurogenesis, and differentiation. This identified multiple distinct neural progenitor populations, as well as gene regulatory networks that control their spatial and temporal identities, and their terminal differentiation into major neuronal subtypes. Integrating these results with large-scale genome-wide association study data, we identified a central role for transcription factors controlling supramammillary hypothalamic development in a broad range of metabolic and cognitive traits. Recurring cross-repressive regulatory relationships were observed between transcription factors that induced prethalamic and tuberal hypothalamic identity on the one hand and mammillary and supramammillary hypothalamic identity on the other. In postnatal animals,

Indexed as

diencephalonDlx1Dlx2gene regulatory networkshypothalamusneurogenesisprethalamussingle cell multiomicstemporal patterningthalamic reticular nucleuszona incerta

Identifiers

PMID39829936
PMCPMC11741371

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.