Evidence map›Paper›PMID 39852148›Full record

ArticleCurrent issues in molecular biology2025

Antiedemic Effect of the Myosin Light Chain Kinase Inhibitor PIK7 in the Rat Model of Myocardial Ischemia Reperfusion Injury.

Dmitry L Sonin, Mikhail S Medved, Asker Y Khapchaev, Maria V Sidorova, Marina E Palkeeva, Olga A Kazakova, Garry V Papayan, Daniil A Mochalov, Sarkis M Minasyan, Ilya E Anufriev and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current issues in molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Dmitry L SoninInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0003-1705-7217
Mikhail S MedvedInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.
Asker Y KhapchaevInstitute of Experimental Cardiology Named after Academician V.N. Smirnov, National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, 121552 Moscow, Russia.ORCID 0000-0001-7889-3337
Maria V SidorovaInstitute of Experimental Cardiology Named after Academician V.N. Smirnov, National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, 121552 Moscow, Russia.ORCID 0000-0003-2540-6325
Marina E PalkeevaInstitute of Experimental Cardiology Named after Academician V.N. Smirnov, National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, 121552 Moscow, Russia.ORCID 0000-0003-4893-4219
Olga A KazakovaInstitute of Experimental Cardiology Named after Academician V.N. Smirnov, National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, 121552 Moscow, Russia.
Garry V PapayanInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0002-6462-9022
Daniil A MochalovInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.
Sarkis M MinasyanInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.
Ilya E AnufrievInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0001-9389-5672
Daria V MukhametdinovaInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0002-7109-1187
Natalia M ParamonovaInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0001-5451-3555
Ksenia M BalabanovaInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.
Anastasia S LopatinaInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.
Ilia V AleksandrovInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0001-8286-8070
Natalya Yu SemenovaInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.ORCID 0000-0003-4069-0678
Anna A KordyukovaLaboratory of Radio- and Optoelectronic Devices for Early Diagnostics of Living Systems Pathologies, The Institute for Analytical Instrumentation, Russian Academy of Sciences, 31-33A Ivana Chernykh Street, 198095 Saint Petersburg, Russia.ORCID 0000-0002-6099-4276
Kirill V ZaichenkoLaboratory of Radio- and Optoelectronic Devices for Early Diagnostics of Living Systems Pathologies, The Institute for Analytical Instrumentation, Russian Academy of Sciences, 31-33A Ivana Chernykh Street, 198095 Saint Petersburg, Russia.
Vladimir P ShirinskyInstitute of Experimental Cardiology Named after Academician V.N. Smirnov, National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, 121552 Moscow, Russia.
Michael M GalagudzaInstitute of Experimental Medicine, Almazov National Medical Research Centre, 15B Parkhomenko Street, 194021 Saint Petersburg, Russia.

Funding

Russian Science Foundation 23-15-00151
6 · The paper itself

Abstract

Myocardial ischemia-reperfusion injury increases myocardial microvascular permeability, leading to enhanced microvascular filtration and interstitial fluid accumulation that is associated with greater microvascular obstruction and inadequate myocardial perfusion. A burst of reactive oxygen species and inflammatory mediators during reperfusion causes myosin light chain kinase (MLCK)-dependent endothelial hyperpermeability, which is considered a preventable cause of reperfusion injury. In the present study, a single intravenous injection of MLCK peptide inhibitor PIK7 (2.5 mg/kg or 40 mg/kg) was found to suppress the vascular hyperpermeability caused by ischemia/reperfusion injury in an in vivo rat model. The antiedemic effect of PIK7 is transient and ceases within 90 min of reperfusion. The early no-reflow detected for the first time after 30 min ischemia in this model of myocardial infarction reduces the area accessible for PIK7. Electron microscopy has shown membrane-bound blebs of endotheliocytes, which partially or completely obturate the capillary lumen, and few capillaries with signs of intercellular gap formation in samples obtained from the center of the early no-reflow zone in control and PIK7-injected rats. Co-injection of PIK7 with NO donor sodium nitroprusside (SNP) increases blood flow in the zone of early no-reflow, while reducing the increased vascular permeability caused by SNP.

Indexed as

ischemic and reperfusion injurymicrovascular hyperpermeabilitymyocardial infarctionmyosin light chain kinaseno-reflowpeptide inhibitor PIK7

Identifiers

PMID39852148
PMCPMC11763459

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.