Evidence map›Paper›PMID 39853686›Full record

ArticleJournal of assisted reproduction and genetics2025

BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis.

Qihui Liang, Chaofeng Wei, Lu Guan, Wen Chen, Shengyong Ding, Haicui Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qihui LiangFirst College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China.ORCID http://orcid.org/0009-0001-9259-9828
Chaofeng WeiFirst College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China.ORCID http://orcid.org/0000-0002-0672-9682
Lu GuanFirst College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China.
Wen ChenFirst College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China.ORCID http://orcid.org/0009-0006-8480-8112
Shengyong DingFirst College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China. dsy200501@126.com.
Haicui WuAffiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250014, Shandong, China. haicui_w@163.com.ORCID http://orcid.org/0000-0003-3313-3697

Funding

Natural Science Foundation of China 82274573Natural Science Foundation of Shandong Province ZR2021MH255Natural Science Foundation of Shandong Province ZR2023QE331Taishan Scholars Project of Shandong Province tsqn202103182
6 · The paper itself

Abstract

purposeTo investigate BMAL1 and BMP6 expressive differences in ovarian granulosa cells (OGCs) of patients with polycystic ovary syndrome (PCOS), explore regulatory relationship, assess their impacts on OGC proliferation and apoptosis, and analyze their correlations with ART outcomes of patients.

methodsA clinical study selected 40 PCOS patients who underwent IVF/ICSI in our hospital from January to October 2022 and 39 controls with male or tubal factor infertility. RT-qPCR and Western blot assessed BMAL1 and BMP6 mRNA/protein levels. The number of oocytes retrieved, 2PN fertilized oocytes, available embryos, and high-quality embryos were compared between groups and analyzed their correlations with BMAL1 and BMP6 expression levels. Cellular experiments were performed by overexpressing or knocking down BMAL1 in KGN cells by plasmid transfection. The dual-luciferase reporter assay was used to identify BMAL1/BMP6 regulatory relationship. CCK-8 and flow cytometry assessed cellular proliferation and apoptosis.

resultsBMAL1 mRNA/protein expression (P < 0.001) in the PCOS group was significantly lower than that in controls, as was the number of high-quality embryos (P = 0.001). Contrastingly, BMP6 (P < 0.001) was significantly higher in the PCOS group. BMAL1 expression levels were negatively correlated with BMP6 (r = - 0.684, P = 0.002) and positively correlated with the number of 2PN fertilized oocytes, available embryos, and high-quality embryos (r = 0.659, P = 0.003; r = 0.623, P = 0.006; and r = 0.738, P < 0.001). Cellular experiments showed that overexpression of BMAL1 significantly decreased relative luciferase activity (P < 0.01). Overexpression of BMAL1 significantly decreased KGN cell apoptosis (P < 0.01) and enhanced proliferation (P < 0.01).

conclusionBMAL1 regulates OGCs proliferation and apoptosis by targeting BMP6, thereby influencing ART outcomes in patients with PCOS. This study might provide molecular factors that indicate ART outcomes and therapeutic targets for PCOS.

trial registrationRegistration number: ChiCTR2100052331; registration date: 2021-10-24.

Indexed as

ARNTL Transcription FactorsBone Morphogenetic Protein 6Granulosa CellsPolycystic Ovary SyndromeReproductive Techniques, AssistedAdultApoptosisCell ProliferationFemaleFertilization in VitroHumansMalePregnancySperm Injections, IntracytoplasmicARNTL Transcription FactorsBMAL1 protein, humanBMP6 protein, humanBone Morphogenetic Protein 6ApoptosisBMAL1BMP6Granulosa cellsPolycystic ovary syndromeProliferation

Identifiers

PMID39853686
PMCPMC11950584

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.